In order to reduce the amount of time required to replicate the very long DNA molecule in eukaryotic chromosomes, DNA replication is initiated at a series of replication origins about 40 kb apart on the linear chromosome and proceeds in both directions. For example, replication in D. ...
These mechanisms involve a complex interplays between DNA accessibility, specific nucleotide sequences (such as AT-richness, a presence of CpG islands and origin G-rich repeated elements, OGREs), nucleosome positioning, epigenetic features, DNA topology and certain DNA structural features (for example,...
For example, in rice, more than 1,600 transgenic T0 lines were required to generate two low-T-DNA-copy lines with nutritionally meaningful iron and zinc elevation13. It is therefore possible that even low-level transgenic expression of mutated IRT1 may be effective for driving substantial ...
Cancer cells often co-opt post-transcriptional regulatory mechanisms to achieve pathologic expression of gene networks that drive metastasis. Translational control is a major regulatory hub in oncogenesis; however, its effects on cancer progression remain poorly understood. Here, to address this, we use...
(b) How it can characterize thousands of genes simultaneously? (c) Give an example of its use. DNA microarray: A DNA microarray is a type of molecular biology technique that is able to measure gene expression. A DNA micro array is also calle...
All molecular biology is based on a central dogma: DNA codes for RNA which then codes for protein (amino acid) production. This concept is universal across all living organisms. Some biological entities will only possess RNA (like viruses, for example) but this p...
For example, findIT_regionRP can accepts the Granges object from calcRP_region to infer influential TF of interesting gene set. findIT_enrichFisher can accept public TF ChIP-seq database to find influential TF of interesting peak set Full size image Table 1 Major FindIT2 functions Full size...
DNAreplication is thought to start 8–12 h post-fertilization [27]. The first point, namely the interpretation of aphidicolin-resistantsynthesis of DNA as unrelated to genomic DNA replication, is based on the currentconcept of DNA replication that implies genomic DNA is replicated (with the except...
As an example, the genome of herpes simplex virus type 1 (HSV-1) encodes seven essential replication proteins (reviewed by Muylaert et al., 2011). These include an ori binding protein (UL9), and six core replication proteins including a DNA polymerase composed by two subunits (UL30 and UL...
For example, the double-stranded DNA sequence for the polypeptide described in the next paragraph is Sign in to download full-size image Fig. 2.1. The structure of DNA. (1) A schematic drawing of the DNA double helix. Two complementary strands (i.e., the base adenine always pairs with ...