A gene vector is a macromolecular assembly designed to deliver therapeutic genetic material to targeted cells by overcoming various barriers in the gene delivery pathway, such as extracellular navigation, cellular membrane passage, intracellular trafficking, and nuclear delivery of the genetic cargo. ...
In subject area: Biochemistry, Genetics and Molecular Biology Generally, it involves the insertion of a foreign DNA fragment, such as a gene, into a genetic vector to form recombinant DNA that can replicate within a host cell, such as the bacterium E. coli. ...
VSG is an example of ‘extreme biology’. VSG is produced from a single active VSG gene, yet comprises by far the most abundant mRNA and protein (~7–10% total) in BF T. brucei. These extraordinarily high expression levels are facilitated by high rates of transcription initiation by Pol ...
nucleic acid substrates (for example, APOBEC1 Km = 0.21 nM for mRNA16) compared to that of wild-type TadA (Km = 830 nM for a tRNA stem17), the inability of wild-type TadA to process DNA and the fact that we evolved TadA-7.10 solely in a Cas-dependent manner2,6,7...
always rely on the availability of living and healthy insects. Although GC-EAD enables convenient screening of active compounds, a large number of bioassay experiments are still required to avoid false positives [9]. For example, a compound can generate an electrical signal but be behaviorally inac...
The shRNAs were not all functionally validated but were given a computationally calculated score for predicated efficiency and specificity. In 2009, Dmitri Wiederschain and colleagues built upon the pLKO vector and made multiple changes, the two most significant of which were the inclusion of the ...
Completion of Vector Preparation and Polymerase Chain Reaction Amplification of egfp SusanCarson, ...Melissa C.Srougi, inMolecular Biology Techniques (Fourth Edition), 2019 TA Cloning One commonly used method, calledTA cloning, takes advantage of an unpaired A-overhang that occurs at the 3′ ends...
McGehee, in Molecular Biology (Third Edition), 2019 1 Properties of Cloning Vectors In principle, any molecule of DNA, which can replicate itself inside a cell, could work as a cloning vector. For convenience in manipulation, the following factors must be considered: 1. The vector should be...
Systems of differential algebraic equations (DAEs) [1] are one particularly common and useful form of mathematical model. These systems are of the general form where F is a function, t is the independent variable, x is the vector of state variables, and x' is the vector of derivatives of...
BioModels Database has become a recognised reference resource for systems biology. It is being used by the community in a variety of ways; for example, it is used to benchmark different simulation systems, and to study the clustering of models based upon their annotations. Model deposition to ...