Evan W., ReynoldsMatthew W., McHenryFabien, CannacJoshua G., GoberChristopher, D. SnowEric M., Brustad
To overcome this issue, an orthogonal enzyme/heme pair consisting of a heme-like cofactor and a complementary heme-binding protein has been developed (Fig. 16c) [148]. In this system, the native heme-transport channel, ChuA, is evolved to import the iron deuteroporphyrin IX (Fe-DPIX) ...
this pair can be expressed in high levels in functional form inE. coli. Indeed, an orthogonal tRNA–synthetase pair evolved from the Mj tyrosyl pair has been used to incorporate more than 20 newamino acidsinto protein inE. coli. Recently, several additional orthogonalE. colipairs we...
Homospermidine synthase, the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis, evolved from deoxyhypusine synthase. Proc. Natl. Acad. Sci. USA 96, 14777–14782, doi: 10.1073/pnas.96.26.14777 (1999). 7. Reimann, A., Nurhayati, N., Backenkohler, A. & Ober, D. Repeated ...
Frugivory evolved multiple times in mammals, including bats. However, the cellular and molecular components driving it remain largely unknown. Here, we use integrative single-cell sequencing (scRNA-seq and scATAC-seq) on insectivorous (Eptesicus fuscus; big brown bat) and frugivorous (Artibeus jamaic...
We established nicotinamide mononucleotide (NMN) as a noncanonical cofactor orthogonal to NAD(P). Here we present the development of Nox Ortho, a reduced NMN(NMNH)-specific oxidase, that completes the toolkit to modulate NMNH:NMNratio together with an NMN-specific glucose dehydrogenase (GDH Ortho)...
coli cell fitness, which sheds light on future work aiming to improve the production of this noncanonical redox cofactor.Finally, we expanded the breadth of our orthogonal NMN(H)-cycling system by integrating it with Saccharomyces cerevisiae. Using the aforementioned production of citronellal as a ...
Brustad and coworkers evolved variants of P450BM3 that are capable of incorporating iron-deuteroporphyrin IX, demonstrating the feasibility of engineering cytochrome P450 variants with orthogonal cofactor specificity [30]. Bordeaux et al. demonstrated that Mb variants incorporating a non-natural Mn-...
However, one of the highest yielding and transferable methods involves utilizing an orthogonal tRNA/tRNA synthetase pair specific to the amber stop codon, as developed by Schultz and coworkers.112 The expression or addition of the tRNA/tRNA synthetase pair is relatively straightforward in both in ...
APOBEC mutagenesis, a major driver of cancer evolution, is known for targeting TpC sites in DNA. Recently, we showed that APOBEC3A (A3A) targets DNA hairpin loops. Here, we show that DNA secondary structure is in fact an orthogonal influence on A3A subst