An enzyme catalyzes the reaction A ⇌ B. The enzyme is present at a concentration of 2 nM, and the Vmax is 1.2 μM s-1. The Km for substrate A is 10 μM. Calculate the initial velocity of the reaction, V0, when the substrate concentration is 20 μM. 相关知识点: 试题来源: ...
Enzyme that catalyzes a peptide-forming reaction from a carboxy component and an amine component, microbe producing the same, and a method of producing a dipeptide using the enzyme or microbe. U.S. Patent. 2013. No. 8,389,241. https://patents.google.com/patent/US838...
a second anticytokine antibody, which is chemically coupled to an enzyme that catalyzes a reaction with later reagents to form a colored compound, is added. Again, the well is rinsed to remove any unbound secondary antibodies and the plate is incubated with a colorless substrate that will ...
l-Aspartic acidis industrially produced by anenzymatic processin whichaspartase(l-aspartateammonia lyaseEC 4.3.1.1) is used. Thisenzymecatalyzes the reversible interconversion betweenl-aspartate andfumarateplus ammonia. Theequilibrium constantof thedeaminationreaction catalyzed by the enzyme is 20mmoll−1...
The biological fitness of microbes is largely determined by the rate with which they replicate their biomass composition. Mathematical models that maximize this balanced growth rate while accounting for mass conservation, reaction kinetics, and limits on
The deletion of pta caused a further metabolic rearrangement. The analysis of genes and enzyme activities related to the central metabolism threw more light on these alterations, most of which were caused by pyruvate/acetyl-CoA accumulation. In general, data on relative gene expression, enzyme activ...
CARNS1 is characterized as an ATP-dependent cytosolic enzyme, which catalyzes the carnosine synthesis from amino acids β-alanine and L-histidine5. It is mostly expressed in skeletal muscles27, some regions of the brain such as the olfactory bulb, and in the heart42. Carnosine is hydrolyzed ...
Focusing on diphenyl diselenide and its derivatives, the more precise findings include mainly those toward the enzyme δ-aminolevulinate dehydratase (δ-ALA-D). δ-ALA-D catalyzes the condensation of two molecules of 5-aminolevulinic acid to porphobilinogen, a monopyrrol precursor of prosthetic gr...
A catalyst of natural origin, such as anenzyme, that can be utilized in the transformation of organic compounds. An enzyme that catalyzes adecarboxylationreaction, generating a molecule of CO2as a byproduct in the enzymatic reaction. A method for generating chiral tertiary carbon stereocenters, usua...
However, there was no increase in the soluble level of valine, leucine or isoleucine indicating that the enzyme is not the rate-limiting step in the pathway. 7.8.3 Aceto hydroxy acid isomeroreductase The enzyme catalyzes a two-step reaction, an alkyl migration and a NADPH-dependent reduction ...