For the vast majority of phosphorylation events, it is not yet known which of the more than 300 protein serine/threonine (Ser/Thr) kinases encoded in the human genome are responsible. Here we used synthetic peptide libraries to profile the substrate sequence specificity of 303 Ser/Thr kinases,...
An atlas of peptide hormone O-glycosylation Fig. 2: Glycosylation of peptide hormones is a widespread phenomenon. Full size image Fig. 3: Topology of O-glycosites in peptide hormones and their predicted functions. We explored the address and message glycosites relative to characterized structural ...
As we assumed that the transcriptome dynamics of NB cells might relate to subtle transitions linked to their original lineage, we generated a single cell transcriptomic atlas of the human SA lineage by integrating key published datasets15,16, with the underlying objective to cover as exhaustively ...
Several classes of irreversible peptidyl inhibitors, such as chloromethanes, fluoromethanes, O-acylhydroxylamines, and vinyl sulfones inhibit the cathepsins B, L, K, and S corresponding to their substrate specificities [24–27]. Some dipeptidyl vinyl sulfones were discovered to be irreversible, ...
Also, the activity of present-day proteins should be easily measurable, in terms of substrate specificities/reaction products (for enzymes) or other biochemical properties (e.g., DNA binding specificity for transcription factors). Possibly, in order to map the position of some of the amino acid...
So et al. observed that CDK5, p35 and Cyclin I mRNA levels are increased in acute myeloid leukemia (AML) patients in The Cancer Genome Atlas dataset (Table1) [109]. Importantly, CDK5 is also frequently deleted in a subset of AML patients, although the prognostic significance of this event...
control. Subsequently, the plates were incubated with anti-human biotinylated IFN-γ detection antibody (Clone: 7-B6-1, 1 mg/ ml, Mabtech), followed by incubation with Streptavidin-AP (1:1000, Mabtech) and BCIP/NBT-plus substrate (Mabtech). Plates were analyzed using the AID ELISPOT Reader...
Atlas of Protein Sequence and Structure 1978, 345–352. Google Scholar Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucl Acids Res 1997, 25(17):3389–3402. 10.1093/nar/...
2). However, Vero cells express high levels of the efflux transporter P-glycoprotein (P-gp) (also known as MDR1 or ABCB1), of which PF-00835231 is a known substrate15 suggesting that the intracellular concentration of PF-00835231 was lower than it initially appeared. Therefore, to evaluate...
Importantly, the ChromHMM-defined chromatin states of CRCs constitute a precise atlas of genome-wide regulatory elements that enables the functional interpretation of ATAC-seq-defined open chromatin regions. Human CRCs are characterized by extensive cellular and molecular heterogeneity. Yet, they also ...