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Bacterialartificial chromosomevectors (BACs) were developed to permit the cloning and stable maintenance of large (100–200kb) pieces of DNA inE. coli. Their stability and ease of handling have made these vectors increasingly popular for whole genome mapping and sequencing projects from microbes, pla...
(1): The MNAzyme will self-assemble on-site by consuming miRNA-21 and Ca2+ions and silencing HSP70 mRNA before photothermal therapy (PTT) treatment. (2) Silencing miR-21 causes Phosphatase and TENsin homolog deleted on chromosome 10 (PTEN) upregulation for PTT sensitization. (3) Ca2+will b...
All in all, artificial gaps degrade the effective performance of most tree reconstruction methods. The inclusion of these positions in the final labeled phylogenies is troublesome as these defects will be passed down to a large number of ancestral sequences, which will be plagued by many false in...
Bacterial artificial chromosome (BAC) libraries continue to be invaluable tools for the genomic analysis of complex organisms. Complemented by the newly and fast growing deep sequencing technologies, they provide an excellent source of information in genomics projects. Results Here, we report the constru...
Carrots (Daucus carota L.), among the most important root vegetables in the Apiaceae family, are cultivated worldwide. The storage root is widely utilized due to its richness in carotenoids, anthocyanins, dietary fiber, vitamins and other nutrients. Carr
with an IS6100-containing non-replicative plasmid. Transposon mutants had stable cointegrates between the transposon vector and the chromosome. Altogether 172 transposon integration sites have been determined by sequencing of the chromosomal inserts, revealing that each integration occurred at a different ...
We measured the query throughput for all four query types mentioned above. To show the robustness of query efficiency on different data, we evaluate on two different chromosome indexes from two different projects. We chose chromosome 2 which is one of the bigger chromosomes and chromosome 22 which...
Some cdtBs also occur on the main chromosome of Fukatsuia, which lacks APSEs; these cdtB homologs are more similar to those occurring in Yersinia rather than those found in Hamiltonella-APSEs (Patel et al., 2019). Recently, cdtBs were found in the genomes of several aphids and drosophil...
The cloning of large segments of DNA (up to a megabase or more) came in 1987 with the invention of YAC (yeast artificial chromosome) cloning by David Burke, Maynard Olson, and others (1987). Two advanced methods for DNA sequencing were reported simultaneously in 1977, by Fred Sanger (70)...