gene therapy programme and concerns in particular a self-replicating vector lacking an antibiotic-resistance gene, comprising a sequence encoding the ccdA protein functionally linked to a first promoter, the sequence of the Cer locus and a heterologous sequence, functionally linked to a second promoter...
This study evaluates the capacity of algae-mediated biological treatment, using the freshwater alga Scenedesmus dimorphus as a representative freshwater species, to deactivate the plasmid pEX18Tc. This plasmid carries the tet antibiotic resistance gene (ARG) which confers resistance to tetracycline. ...
Gene to be regulated for expression: In addition to the perfect expression of the gene inside the targeted cell, the amount of gene expression should be able to regulate according to the circumstance. 4. Lower immunity: A vector with high immunity will be easily eliminated by the immune system...
L. The Escherichia coli Cpx envelope stress response regulates genes of diverse function that impact antibiotic resistance and membrane integrity. J. Bacteriol. 195, 2755–2767 (2013). Article CAS PubMed PubMed Central Google Scholar Guest, R. L., Wang, J., Wong, J. L. & Raivio, T. ...
In this project we have analysed the use of an intron-containing neomycin phosphotransferaseII – nptII – gene. The advantage of this construct is that only eukaryotic organisms will be able to process this gene. Accordingly, the theoretical risk of horizontal gene flow of antibiotic resistance g...
Pärnänen for helpful discussion on metagenomic data processing and calculating antibiotic resistance gene abundances from metagenomic data. Additionally, we thank S. Blomquist for help with running analysis pipelines via the Commonwealth Computational Cloud for Data Driven Biology (C3DDB) cluster. ...
(HGT), such as prophage sequences. Many of the antibiotic resistance genes were absent in very closely related genomes. The genesaadA1,sat2,dfrA1andblaTEM-1-likeformed a gene cluster recently acquired, explaining the G + C content profile of the region (Fig.1). Moreover, 22 ...
In conclusion, GLP-1 promoter and L-cell could be potential candidates for diabetes gene therapy agents.Background Diabetes mellitus is characterised by metabolic disorders and abnormally high blood glucose, which are caused by the destruction of the β-cells of the pancreas, insulin resistance and...
They are classified into three categories: antibiotic resistance gene, anti-metabolic marker gene, and herbicide resistance gene, among which antibiotic resistance gene is the most commonly used (Aaronson, 2000). Reporter genes are generally considered to be quantifiable by estimating the amount of ...
Although the original ColE1 plasmid was once used directly as a vector, most modern ColE1-based vectors contain a range of artificial improvements. First, the genes for colicin E1 were removed, since these are obviously not needed. Next, an antibiotic resistance gene was added, which makes the...