All RNA samples were analyzed for quality on an Agilent 2100 Bioanalyzer. The RNA samples were processed utilizing Illumina’s TruSeq small RNA sample preparation protocol for small RNA library generation (Part# 15004197 Rev. F, Cat# RS-200-9002DOC). The subsequent sequencing was performed on ...
RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA). Then the libraries were constructed using the VAHTS Universal V6 RNA-seq Library Prep Kit according to the manufacturer’s instructions. Transcriptome sequencing and analysis were conducted by OE Biotech Co....
可于变性琼脂糖凝胶中对总RNA样本(2-5 µg)电泳分析,并使用溴化乙锭染色分析,或通过 Agilent 2100 Bioanalyzer™ 设备进行分析(图1)。完整的总RNA样本中28S与18S核糖体RNA条带会显示出2:1的信号强度比率。核糖体比值显著低于2:1通常意味着发生了降解。 图1a.完整RNA与降解RNA之间的对...
RNA quality was evaluated by microfluidic capillary electrophoresis on an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA) using Agilent RNA 6000 Pico kit. Chips were prepared and loaded according to the manufacturer's instructions. Isolated RNA was stored at -80°C for no longer ...
cDNA libraries were quantified using an Agilent 2100 Bioanalyzer and sequenced with an Illumina NovaSeq S4. Quality control and filtering We filtered cells using highly stringent quality control (QC) metrics. In brief, we discarded potential doublets using the R package scrublet29 for each individual...
(Promega, Madison, WI, USA), according to the manufacturer’s instructions. The quantity and quality of each extract were then determined with an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA, USA). We generated 24 libraries, corresponding to 2 dormancy phases × ...
Samples were extensively treated with RNAse-free DNase using the DNA-free™ Kit (Ambion, Inc., Austin, TX) and the quality of the sample was ascertained using an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA). Each condition was done in duplicate using two separate animals....
This approach is applied to a large collection of electrophoretic RNA measurements recorded with an Agilent 2100 bioanalyzer to extract an algorithm that describes RNA integrity. The resulting algorithm is a user-independent, automated and reliable procedure for standardization of RNA quality control that...
RNA was quantified by spectrophotometry, and its integrity verified with a 2100 Bioanalyzer (Agilent). The RNA (450 ng) was treated with the Turbo-DNA free kit (Ambion), and 350 ng of the treated RNA was reverse-transcribed with SuperScript III (Invitrogen) using an oligo(dT) primer ...
Bioanalyzer 2100 (Agilent) d. Standard laboratory equipment i. Centrifuge ii. pipettes 2. Reagents a. Custom oligos (5′ > 3′; we had them synthesized by IDT, but it could be done by many vendors). Oligos marked with * were HPLC purified. Dissolve each stock oligo to 100μM in 10 ...