the Bayesian framework, which allows selecting a final feature combination corresponding to models with high posterior probability.This approach is applied to a large collection of electrophoretic RNA measurements recorded with an Agilent 2100 bioanalyzer to extract an algorithm that describes RNA integrity...
可于变性琼脂糖凝胶中对总RNA样本(2-5 µg)电泳分析,并使用溴化乙锭染色分析,或通过 Agilent 2100 Bioanalyzer™ 设备进行分析(图1)。完整的总RNA样本中28S与18S核糖体RNA条带会显示出2:1的信号强度比率。核糖体比值显著低于2:1通常意味着发生了降解。 图1a.完整RNA与降解RNA之间的对...
RNA quality was evaluated by microfluidic capillary electrophoresis on an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA) using Agilent RNA 6000 Pico kit. Chips were prepared and loaded according to the manufacturer's instructions. Isolated RNA was stored at -80°C for no longer ...
All RNA samples were analyzed for quality on an Agilent 2100 Bioanalyzer. The RNA samples were processed utilizing Illumina’s TruSeq small RNA sample preparation protocol for small RNA library generation (Part# 15004197 Rev. F, Cat# RS-200-9002DOC). The subsequent sequencing was performed on ...
followed by end reparation and ligation to adaptors. The quantity and quality of the cDNA libraries were assessed using an Agilent 2100 BioAnalyzer. Finally, the libraries were sequenced on the BGISEQ-500 with 50 single-end reads. Sequencing reads that contained adaptors were of low quality, or ...
Library size distribution was checked with an Agilent 2100 bioanalyzer, and quantity was determined using qPCR. Libraries were sequenced on an Illumina HiSeq 2500 using a 50-cycle rapid-run kit or an Illumina NextSeq instrument using a 75-cycle high-throughput kit. Read quality was confirmed ...
the Bayesian framework, which allows selecting a final feature combination corresponding to models with high posterior probability.This approach is applied to a large collection of electrophoretic RNA measurements recorded with an Agilent 2100 bioanalyzer to extract an algorithm that describes RNA integrity...
We used deep sequencing to characterise mtDNA deletion breakpoints. Briefly, selected amplicons ( ≤ 9 kb), were purified using Agencourt AMPure XP reagent and assessed for concentration and quality using an Agilent 12000 DNA kit on a Bioanalyzer (Agilent 2100, Agilent Technologies). 100 ...
The quality of RNA was tested by electrophoresis using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Total RNA was labeled with Cyanin-5 CTP by linear amplification using a Low RNA Input Fluorescent Linear Amplification Kit (Agilent Technologies) as specified by the ...
RNA was quantified by spectrophotometry, and its integrity verified with a 2100 Bioanalyzer (Agilent). The RNA (450 ng) was treated with the Turbo-DNA free kit (Ambion), and 350 ng of the treated RNA was reverse-transcribed with SuperScript III (Invitrogen) using an oligo(dT) primer ...