Binding capacity at pH 6.0 relative to binding capacity at pH 7.4 is also depicted (top row). For binding at acidic pH, red indicates a greater than threefold impairment in kd at pH 6.0, green indicates a less than threefold impairment, and white indicates no data. These data are ...
Here we report that V-domain immunoglobulin suppressor of T cell activation (VISTA) engages and suppresses T cells selectively at acidic pH such as that found in tumour microenvironments. Multiple histidine residues along the rim of the VISTAextracellular domain mediate binding to the adhesion and ...
Leaves of the carnivorous sundew plants (Droseraspp.) secrete mucilage that hosts microorganisms, but whether this microbiota contributes to prey digestion is unclear. We identified the acidophilic fungusAcrodontium crateriformeas the dominant species in the mucilage microbial communities, thriving in multi...
英文名称Acidic alpha-naphthol acetate esterase staining kit (ANAE method) 中文名称酸性α-乙酸萘酚酯酶染色试剂盒(ANAE法) 保存条件4℃避光保存,有效期6个月。 产品介绍酯酶主要分为非特异性酯酶(non-specific esterase)、酯酶(lipase)、胆碱酯酶(choli-esterase)。每一种酯酶常能水解许多不同的底物,多种不同的...
immobilized metal affinity chromatography (IMAC), which employs Fe3+ or Ga3+-chelated solid supports to selectively bind and enrich phosphopeptides (derivatization of other acidic groups by methyl esterification prevents non-specific bindings and has been used to detect more than 1000 phosphopeptides and...
PbChi74 displayed an acidic pH optimum of 4.5 and a temperature optimum of 65??C. The enzyme showed high activity toward colloidal chitin, glycol chitin, N-acetyl chitooligosaccharides, and p-nitrophenyl N-acetyl ??-glucosaminide. PbChi74 hydrolyzed colloidal chitin to yield N-acetyl chito...
PicrophilusandFerroplasmaare found in acidic sites. These microorganisms generally require intracellular neutral pH but the mechanism of homeostasis in acidophiles is not well understood. The major factor that contributes to intracellular pH is membrane impermeability which controls the proton influx inside...
The molecular characterization using 16s rDNA sequencing revealed the identity of bacteria as Acinetobacter sp. However, this new acid-tolerant PSB is the first report from Kerala, which could be a potential biofertilizer for acidic soils of Kerala. 展开 年份: 2015 ...
While MHV is considered to be stable in the pH range of 5–7.4 at 37 °C and 3–10 at 4 °C (Casanova et al. 2009; Daniel and Talbot 1987), the stable pH range for TGEV is 5–7 at 37 °C and 5–8 at 4 °C. In acidic pH, reversible acid denaturation of RNA through ...
It is distinctive that the acidity of the lysosome lumen engrossingly creates an optimal working environment for over 60 lysosomal hydrolases, most of which have acidic pH optima (between 4.5 and 5.5). This acidic characteristic has been attributed to the activity of vacuolar-type H+-ATPase (V...