A low level of Bt1-specific proteins was detected at 10 d after pollination (DAP) and increased to a plateau at 16 DAP. At the same time, the ratio of slow to fast-migrating forms of the protein decreased. During endosperm fractionation by differential centrifugation and membrane sedimentation...
proteins (Supplementary Fig.6g). These results demonstrated that the zein protein level itself is not associated with endosperm texture in this study. We then examined starch synthesis in NILW64A and NILA619 and found the expression of all starch synthetic genes and the total starch and amylose...
Reference work 2016, Encyclopedia of Cell BiologyR.R. Wise Chapter Analysis of Glycans; Polysaccharide Functional Properties 2.18.2.1 Introduction Starch, a polymer of d-glucose, is synthesized and stored in plastids as highly ordered particles (‘granules’). Chloroplasts serve as short time store ...
Book series2011, International Review of Cell and Molecular Biology Shingo Takagi, ... Kosei Iwabuchi Explore book 4 Overview of Recent Investigation on Plastids–Cytoskeleton Interactions Plant cells bear a plant-specific double-membrane-bounded compartment: plastids. Since many aspects of dynamic behavi...
Then, the sections were washed 5 times with PBS (2 min for each wash), and labeled with protein A Gold 10 nm (1:50; Dept. of Cell Biology, UMC Utrecht, the Netherlands) for 30 min at room temperature. After several washes with PBS, the sections were fixed in 1% (w/v) ...
We then explored the effect of nitrogen level (0, 210, and 315 kg N ha(-1)) on starch granule size and tuber greening of three commercial cultivars. Results showed that chlorophyll content of tuber skin increased (5-8 times) in a quadratic manner with increasing duration of light exposure...
Then, the sections were washed 5 times with PBS (2 min for each wash), and labeled with protein A Gold 10 nm (1:50; Dept. of Cell Biology, UMC Utrecht, the Netherlands) for 30 min at room temperature. After several washes with PBS, the sections were fixed in 1% (w/v) ...