amyloid plaques (Extended Data Fig.2a). To assay microglial autophagy in vivo, we crossed 5xFAD mice with an autophagy reporter line expressing green or negative (−) for Clec7a, a marker for DAM19, from
A strategy for immunizing against amyloid plaques using display technology. The strategy includes methods, agents, and pharmaceutical compositions for vaccination against plaque forming diseases (e.g., Alzheimer's disease) that rely upon presentation of an antigen or epitope on a display vehicle. The...
The implementation of this model in research might lead to a better understanding of disease pathophysiology, a revision of the current clinical taxonomy and accelerated development of strategies to prevent and treat AD.This is a preview of subscription content, access via your institution ...
On the other hand, abnormal increase in Aβ levels, even before its accumulation in plaques, appears to be toxic in vitro and in vivo [3]. Consistent with this peptide playing a key pathological role in AD, mutations in either APP or its secretases are a well established cause for the ...
Initially, the amyloid plaques have a diffuse, amorphous appearance and are spherical in shape. Over time, the innate immune system mounts a response to these plaques. The response includes microglia (a type of immune cell within the brain), activated astrocytes (astrocytes within the brain ...
Methods that can expand the knowledge about how the proteins are incorporated in plaques are described. Top-down methods to analyze post-mortem tissue and bottom-up approaches with the potential to provide molecular insights on the organization of plaque-like particles are compared. Finally, a ...
In this section we summarise these data and how the dominance of this hypothesis arose. Putative evidence in support of the hypothesis Using the amino acid sequence corresponding to Aβ [9], the major constituent of amyloid plaques in AD, a precur- sor gene cDNA to Aβ (the amyloid ...
Classification of amyloidosis: misdiagnosing by way of incomplete immunohistochemistry and how to prevent it. Acta Histochem. 2006;108(3):197–208. Article PubMed Google Scholar Barreca A, et al. Immunohistochemical typing of amyloid in fixed paraffin-embedded samples by an automatic procedure: ...
Marina Scharin-Mehlmann3, Daniel F. Gilbert4, Oliver Friedrich4, Teja Grömer1, Johannes Kornhuber1, Roland Lang5,† & Juan Manuel Maler1,† Amyloid-β (Aβ) peptides are the main components of the plaques found in the brains of patients with Alzheimer's disease...
Fresh S. Typhimurium biofilms were prepared on glass coverslips for 72 h, comparing cultures incubated with 6A, 3H3, or a polyclonal anti-CsgA serum that is known to completely inhibit biofilm formation. In these experiments, the biofilms were very gently washed prior to imaging to prevent ...