49.Bendit EPt, Eriksen N. Chemical similarity among amyloid substances associated with long standing inflammation. Lab Invest 1971;26(6) 615-625. 50.Levin M, Pras M, EC Franklin EC. Immunologic studies of the major nonimmunoglobulin protein of amyloid. I. Identification and partial characterizatio...
Plates were incubated in a microplate reader (FLUOstar OMEGA, BMG Labtech) for ~100 hours at 37 °C with 700 r.p.m. double orbital shaking. Fluorescent measurements were recorded every 15 minutes, with excitation wavelength λex = 440 nm and emission wavelength λem =...
These initiatives will, together with novel assays produced under rigorous QC measures and run on fully automated analytical instruments, improve the quality and reduce between-lab variability, thereby allowing uniform cutoff levels for diagnosis and a more widespread use of CSF biomarkers in the ...
Chang’s Lab C57BL/6 J KOATECH http://www.koatech.co.kr/ Software and algorithms ImageJ NIH https://imagej.nih.gov/ij/ Fiji NIH https://imagej.nih.gov/ij/ Prism Software GraphPad Software, Inc., La Jolla, USA https://www.graphpad.com/scientific-software/...
Next, PVE-lab software was used to segment grey matter (GM), white matter (WM) and cerebrospinal fluid (CSF), as well as to delineate volumes of interest (VOIs) based on the Hammers atlas [23, 24]. The following grey matter regions were used as target regions: medial and lateral ...
To determine the cytotoxicity of siRNA-encapsulated PLGA NPs, cell viability was measured using the EZ-Cytox cell viability assay kit (Daeil Lab Service Co. Ltd.) in accordance with the manufacturer’s instructions. Cell viability was quantified by measuring the absorbance at 540 nm using a Sun...
The novel object recognition task consisted of a habituation phase followed by training and testing, as used in our lab previously6. Mice were habituated in an open field testing box for 10 min on 3 consecutive days. On the fourth day, 2 identical wooden blocks (Premium wooden building blo...
150 μM in 50 mM Tris-HCl, pH 7.5, with 0.1 M NaCl, supplemented with either 5 mM EDTA or 5 mM monitored by bottom reading the fluorescence intensity CinaCa lP2.OTLhAeRksitnaertimcsicorfotphlaioteflraevaindeTr (ThT) (BMG binding was Labtech), as previously described36,57...
Data were analyzed and histograms were created by means of OriginPro (OriginLab) software. Quantitative imaging measurements. The nanomechanical measurements were obtained by using a JPK Nanowizard III atomic force microscope (JPK Instruments AG, Germany). We chose CSC cantilevers (μM asch,...
Slides were incubated overnight with rabbit polyclonal antiserum against purified mouse SAA (generated by Lablogix corporation), washed, and incubated with fluorescein-isothiocyanate (FITC)-conjugated secondary antibody (Jackson ImmunoResearch Laboratories, West Grove, PA). Slides were washed with PBS, ...