each testing a different condition of amylase. The first test was to simply study the reaction betweensalivaand amylase and note the reaction rates. The second test was to see if increasing the pH would decrease the reaction rate or halt it all together. Saliva was collected, diluted, and t...
What is the meaning of this? Why is it necessary for the stomach contents to be acidic? How does the stomach protect itself from digestion? Describe the general functions of saliva. (a) What is the function of the epiglottis? (b) How does it work? What separates the stomach from the...
As for AMY2 (4), three substrate binding subsites are occupied by a molecule of component 2 (a pseudotrisaccharide derived from acarbose after cleavage of a glucose unit from the reducing end) meaning that unit A to C occupies subsites -1 to +2. The starch granule-binding surface site ...
On starch hydrolysis test (amylase test), how would you expect both positive and negative results to be affected if you were to add glucose to the medium? A) What is the general meaning (not just as it applies to enzymes) of the phrase optimum conditions? B) Why do most enzymes exhibit...
Tests results for amylase activity on\npolysaccharides, and amylase activity on disaccharides is compared to a\nnormal range of 30 to 120 minutes for disaccharides, and 4 to 8 hours for\npolysaccharides (meaning: 50 to 250 mg/dl of glucose detected in a\ndisaccharide test sample within 30 ...
Blue-starch Phadebas amylase test (this work). The VHH Structures The VHH polypeptidic chains are not complete and are visible in density from residues 2 to 25 and 28 to 111 for AMB7 VHH (123 residues), from 1 to 111 for AMD9 VHH (118 residues), and from 2 to 112 for AMD10 VHH...
Residual starch iodine test: A sample of the fermentation broth is centrifuged in 2 ml plastic centrifuge tubes. The supernatant is decanted and the tube containing the pellet is placed in an ice bath. Several drops of 0.025N iodine solution (0.1N iodine from VWR Cat. No. VW3207-1 diluted...
The net charge of one subunit of the inhibitor 0.19 was increased by three basic charge units with seven amino acid substitutions meaning that six more basic charge units increase per dimeric form protein [45]. This charge difference might result in the relative mobility of gel electrophoresis ...