The major concern in an enzymatic process is the instability of the enzyme under repetitive or prolonged use and inhibition by high substrate and product concentration. Immobilization is a very effective alternative in overcoming problems of instability and repetitive use of enzymes. Entrapment method ...
Annexin V Unlabeled Reagent BioVision-1005-5000 10X Annexin V Binding Buffer BioVision-1006-100 Caspase-3 Substrate DEVD-AFC BioVision-1007-1000 Caspase-3 Substrate DEVD-AFC BioVision-1007-200 Caspase-3 Substrate DEVD-pNA BioVision-1008-1000 使用...
There are different methods to investigate the α-amylase inhibitory activity, such as measurement of fecal sugar and extraction of the enzyme to measure its available concentration using a substrate or gelatinizing starch[25]. In Western medicine, acarbose is used as an α-glucosidase inhibitor, ...
The blocking group in BPNPG7 prevents hydrolysis of this substrate by exo-acting enzymes such as amyloglucosidase, α-glucosidase, and β-amylase. When the substrate is cleaved by endo-acting α-amylase, the nitrophenyl oligosaccharide is immediately and completely hydrolyzed to p-nitrophenol and ...
and amylopectin, randomly cutting the internal of the sugar chain α- 1, 4-chain. Therefore, it is characterized by a sharp decline in the viscosity of the substrate solution and the disappearance of the iodine reaction. The final product is mainly glucose in the decomposition of amylose. In...
When alpha-Amylase acts on starch, the viscosity of the solution gradually decreases while the reducing power increases. As the substrate concentration decreases, the product concentration increases, which can lead to partial inactivation of the enzyme and a gradual decrease in reaction rate until the...
The substrate specificity and end product analyses confirm that it is a calcium-dependent α-amylase enzyme, which exhibits its maximum activity at 31°C and at pH between 6.5 and 7.5. The Amy1 protein breaks down mainly starch, is also able to cleave glycogen and dextrin, and exhibits no ...
Pancreatin, an enzyme mixture isolated from pancreas, produced from the same substrate chiefly maltose and maltotriose at 20–50 °C and maltose at 70 °C, whereas malt amylase gave mainly maltose, accompanied by glucose at 20 °C, and small amounts of maltotetraose and maltopentaose at 70 ...
improved binding of a particular type of substrate, improved specificity towards a substrate, and/or improved specificity with respect to cleavage (hydrolysis) of substrate. An amino acid sequence is considered to be X% homologous to the parent α-amylase if a comparison of the respective amino...
using the BCA Reducing Ends Assay. Briefly, 1 part Copper (II) Sulfate Solution with 49 parts Bicinchoninic acid solution was mixed prior to the start of the assay. 1% potato amylopectin was used as a substrate and kept at the assay temperature for 10 minutes prior to the addition of ...