The purpose of the current study is to isolate a heavily amylase-producing bacteria of the genus Bacillus from soil samples, optimize the production of the enzyme, purify it, and evaluate its activity against biofilm-producing bacteria. A total of 12 soil samples were collected and screened for...
Considering the effect of pH, most of the enzymes from the tested fish species revealed high stability in a broad pH range (from 5.0 to 11.0) with enzyme activity up to 110% (Fig.4b). However, in the case ofP. gonionotus, the relative amylase activity decreased and remained at 55% at...
The enzyme was moderately inhibited (44.7%) by NaCl and KCl (0.105 U/mL) with minimum inhibition (14.8%) observed with SDS. Molecular weight calculation and amylase confirmation in protein sample was done by SDS–PAGE and Zymography. Calculated molecular weight was 56 kDa. Alkaline ...
Enzyme Activity:α-Amylase, β-Amylase EC Number:α-amylase:3.2.1.1, β-amylase:3.2.1.2 CAZy Family:GH13, GH14 CAS Number:α-amylase:9000-90-2, β-amylase: 9000-91-3 Synonyms:α-amylase:alpha-amylase, β-amylase:beta-amylase Source:Hordeum vulgare ...
Industrial processes for starch hydrolysis to glucose rely on inorganic acids or enzyme catalysis. The use of enzymes is preferred as it offers a number of advantages including improved yields and favourable economics. Enzymatic hydrolysis allows greater control over amylolysis, the specificity of the ...
terminated, and the phenolate color developed by the addition of an alkaline solution is measured at 400 nm. Amylase activity is expressed in terms of Ceralpha units; 1 unit is defined as the amount of enzyme required to release 1 µmolp-nitrophenyl (in the presence of excess quantities ...
Rhodanine-benzamides as potential hits for α-amylase enzyme inhibitors and radical (DPPH and ABTS) scavengers Article 06 March 2024 New Hybrid Hydrazinyl Thiazole Substituted Chromones: As Potential α-Amylase Inhibitors and Radical (DPPH & ABTS) Scavengers Article Open access 05 December 2017 ...
for 45 min at 37 °C in the dark. The solvent was removed by evaporation to dryness. Tryptic hydrolysis (0.5 mL, enzyme-to-substrate ratio 1:50, 0.04 mol/L urea in 0.1 mol/L Tris–HCl, pH 7.8) was performed for 18 h overnight at 37 °C in the dark. The reaction was stopped ...
From the above experimental results, it can be seen that the n-butanol fraction has a higher inhibitory enzyme activity, and it is inferred that the high-polarity solvent extraction has a better inhibitory rate for the enzyme. The literature shows that cinnamon is extracted with a high-polarity...
2.6. Analysis of AmyZ1 activity, invertase activity, and pullulanase activity The activity of AmyZ1 was determined using the modified dinitrosalicylic acid (DNS) assay method at 40 °C [5]. One unit of AmyZ1 activity was defined as the amount of enzyme needed to release 1 μmol of reduc...