Ten new proansamycin B congeners (1–10) together with one known (11) were isolated and characterized on the basis of 1D and 2D NMR spectroscopic and HRESIMS data from theAmycolatopsis mediterraneiS699 ΔPM::rifR+rif-orf19mutant. Compounds8and9featured with six-membered ring and five-...
The gene rif orf14 in the rifamycin biosynthetic gene cluster of Amycolatopsis mediterranei S699, producer of the antitubercular drug rifamycin B, encodes a protein of 272 amino acids identified as an AdoMet: 27- O-demethylrifamycin SV methyltransferase. Frameshift inactivation of rif orf14 generat...
mediterranei S699 and A. mediterranei LBGA 3136. However, there are several other strains of A. mediterranei which also produce rifamycins. In order to detect the variability in the rif gene cluster among these strains, several strains were screened by PCR amplification using oligonucleotide primer...
The development of a facile host–vector strategy for genetic engineering of the rifamycin PKS in the producing organism, Amycolatopsis mediterranei S699, is described here. The genes encoding all 10 modules of the rifamycin PKS were replaced with a hygromycin-resistance marker gene. In a similar ...
A genomic and bioactivity informed analysis of the metabolome of the extremophile Amycolatopsis sp. DEM30355 has allowed for the discovery and isolation of the polyketide antibiotic tatiomicin. Identification of the biosynthetic gene cluster was confirme
mediterranei S699, U32 probably employs the same two alternatives to cope with rifamycin cytotoxicity, that is, several mutations in RNA polymerase β-subunit (AMED_0656) to lower its affinity for rifamycins and a rifamycin exporter RifP (AMED_0633) to prevent the intracellular accumulation of ...
Amycolatopsis pretoriensis Amycolatopsis lexingtonensis Amycolatopsis balhimycina DSM 44591T Amycolatopsis mediterranei F1-24 Amycolatopsis mediterranei RB Amycolatopsis mediterranei NRRL B-3240 Amycolatopsis mediterranei U32 Amycolatopsis mediterranei S699 AMD AMN Amycolatopsis orientalis HCCB10007 Amycolatopsis sp....
Medium optimization for enhanced production of Rifamycin B by Amycolatopsis mediterranei S699: combining a full factorial design and a statistical approach. Process Biochemistry . 2008; 43 (9):954–960.Ma FX, Kim JH, Kim SB, Seo YG, Chang YK, Hong SK, et al. Medium optimization for ...
Amycolatopsis mediterranei S699 produces rifamycin B and successors of this strain are in use for the industrial production of rifamycin B. Semisynthetic derivatives of rifamycin B are used against Mycobacterium tuberculosis that causes tuberculosis. Although the rifamycin biosynthetic gene ...
Amycolatopsis mediterranei S699Pentangular polyphenolsTopoisomerase II alpha inhibitormin-PKSTen new pentangular polyphenols, namely amexanthomycins A–J ( 1– 10) were isolated from the strain Amycolatopsis mediterranei S699∆ rifAconstructed by deleting the polyketide synthase genes......