Here, we present an integrated, amplification-free CRISPR-Cas12a detection platform combined with a smartphone-based point-of-care (POC) device for rapid, sensitive, and portable quantification of P.g. By employing a multiplex crRNA strategy, the platform achieves a detection sensitivity of 12.5pg...
Amplification-free digital gene expression profiling from minute cell quantities. Nat. Methods. 2010; 7 :619–621. [ PMC free article ] [ PubMed ]Ozsolak F, Ting DT, Wittner BS, Brannigan BW, Paul S, Bardeesy N, Ramaswamy S, Milos PM, Haber DA: Amplification-free digital gene ...
Comprehensive analysis of the resulting data shows that amplification-free Hi-C preserves higher complexity of chromatin interaction and lowers sequencing depth for the same number of unique paired reads. For human cells which have a large genome, SAFE Hi-C recovers enough ligated fragments for ...
«An accurate amplification-free CRISPR/Cas12a-based assay for GES β-lactamase detection» «用于检测GES β-内酰胺酶的精确无扩增CRISPR/Cas12a分析» 相关领域 清脆的 生物 计算生物学 微生物学 遗传学 基因 网址 https://doi.org/10.1016/j.ijantimicag.2025.107506 ...
Our target amplification-free collateral-cleavage-enhancing CRISPR-CasΦ method (TCC), with a detection limit of 0.11 copies/μL, demonstrates enhanced sensitivity compared to qPCR. It can detect pathogenic bacteria as low as 1.2 CFU/mL in serum within 40min.#Rapid diagnosis of bloodstream ...
Cover Picture: A Rapid, Amplification‐Free, and Sensitive Diagnostic Assay for Single‐Step Multiplexed Fluorescence Detection of MicroRNA (Angew. Chem. Int. Ed. 34/2015)Cover Picture: A Rapid, Amplification‐Free, and Sensitive Diagnostic Assay for Single‐Step Multiplexed Fluorescence Detection of...
Related to amplification:DNA amplification am·pli·fi·ca·tion (ăm′plə-fĭ-kā′shən) n. 1.The act or result of amplifying, enlarging, or extending. 2. a.An addition to or expansion of a statement or idea. b.A statement with such an addition. ...
An amplification-free Cas12a-based method was proposed to quantifyL. plantarum.Thetrans-cleavage rate was improved with multiple crRNAs and chemical additives.The quantitative performance was comparable to qPCR for detectingL. plantarum.关键词: Food fermentation Microbial quantification CRISPR/Cas12a Ampli...
It will be marketed under the name "TruePrime Cell-Free DNA Amplification Kit." This new method was recently presented to international media in Madrid by SYGNIS' scientific advisors Prof. New tool for the detection and amplification of cell-free DNA in blood The design was based on the teles...
that this is a highly sensitive and amplification-free method of quantifying circulating miRNAs in blood serum and can potentially be used as a clinical monitoring tool, with applications not only for PCa but also for a wide range of cancers and other diseases....