DNA replication in bacteria is accomplished by a multicomponent replicase, the DNA polymerase III holoenzyme (pol III HE). The three essential components of the pol III HE are the α polymerase, the β sliding clamp processivity factor, and the DnaX clamp-loader complex. We report here the ass...
This is accomplished by way of an informatics based method that can compare the genetic fingerprint of the fetal DNA found in maternal plasma to the genetic fingerprint of the alleged father.Allison RyanStyrmir SigurjonssonMilena BanjevicGeorge Gemelos...
In the denaturation process, the two intertwined strands of DNA separate from one another, producing the necessary single-stranded DNA template for replication by the thermostable DNA polymerase. In the next step of a cycle, the temperature is reduced to approximately 40–60°C. At this ...
Summary of the 5' RACE System The 5' RACE System is a set of prequalified reagents intended for synthesis of first strand cDNA, purification of first strand products, homopolymeric tailing, and preparation of target cDNA for subsequent ...
Once DNA is isothermally unwound and stabilized, two oligonucleotide primers hybridize to the 5′ and 3′ borders of the target sequence and a DNA polymerase extends the annealed primers by adding deoxynucleotide-triphosphates (dNTPs) to generate double-stranded amplification products (Fig. 1), in...
DNA content was normalised to that of long interspersed elements (LINE1), a repetitive element for which copy number per haploid genome is similar in both the normal DNA sample and DNA from cancer cells. Primers were designed by Primer express 3.0 software (Applied Biosystems, Foster City, CA...
The authors describe a colorimetric method for the determination of Hg(II) ion. It is based on the color change from red to colorless as displayed by gold nanoparticle (AuNP) modified with thymine - rich DNA. Signal amplification is accomplished by free strand displacement recycling. In this ...
(b) converting the nucleic acid sequence of interest into a first hairpin DNA structure by ligating a first single stranded nucleic acid to the 5′ end of the upper strand of the template nucleic acid, ligating a second single stranded nucleic acid, which is non-complementary to the first ...
Detection of microorganisms can be accomplished by amplifying a segment of DNA that is specific to the type of microorganism to be detected followed by detection of the amplified DNA. If the DNA is present at detectable levels after amplification, this indicates the presence of the microorganism of...
Antibodies which are specific to a thermostable DNA polymerase can be used to reduce or eliminate the formation of non-specific products in polymerase chain reaction methods. These antibodies and othe