透析结束后,将血浆在4℃下14000g离心30min,将上清利用AKTA蛋白纯化系统(美国Cytiva公司)和Protein A/G亲和层析柱(美国Cytiva公司)进行纯化。用0.1M甘氨酸溶液(pH2.7)洗脱目的抗体,然后迅速滴加1M Tris‑HCl缓冲液(pH9.0)将抗体pH值调至7.2～7.4。分装1mg/mL/管,‑80℃保存。
Next, the slices were observed using a microscope (Olympus). 2.18. Measurement of ATP production A total of 10 mg myocardium was homogenized in 100 μl of ATP assay buffer, centrifuged, and then the supernatant was deproteinated. The ATP contents of samples were measured with the ATP Assay...
Figure 3.Cr(VI) interfered with the energy balance through inhibiting the AMPK/PGC-1α signaling pathway in the liver (A) AMPK and PGC-1α protein bands; (B) AMPK and PGC-1α protein expression levels; (C) CS activity; (D) MDH activity; (E) ATP content. With the column data, the...