摘要: Correction: Fenofibrate Improves Renal Lipotoxicity through Activation of AMPK-PGC-1α in db/db Micearticlehttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0105921关键词: Science(General DOI: 10.1371/journal.pone.0105921 ...
AMPK, AMP-activated protein kinase CaMKK尾, calmodulin-dependent kinase kinase 尾 ACC, acetyl-CoA carboxylase HMGCR, 3-hydroxy-3-methyl-glutaryl-CoA reductase SREBP, sterol regulatory element binding protein PGC1伪, peroxisome proliferator-activated receptor 纬 co-activator 1伪 NAD+, nicotinamide aden...
对蛋白质裂解物进行sds-page,电转移到pvdf膜上并用以下抗体进行探测:paccα-ser79、accα(cellsignaling,美国马萨诸塞州丹佛斯)、ucp1、ucp3、lamp2b(abcam;英国剑桥)、pgc1α、pgc1β、grp94(santa cruz biotechnology,美国德克萨斯州达拉斯)、β-肌动蛋白、α-微管蛋白(sigma-aldrich;美国密苏里州圣路易斯)和...
只小鼠/组),持续6天后,小鼠BAT中代表性UCP1、UCP3、PGC1α和PGC1β蛋白质印迹图像(a)及 其表达定量(b)。α‑微管蛋白用作蛋白质上样的对照。当样品上样到同一凝胶上,但不是并 排上样时,在免疫印迹上插入一条黑线。(c,d)基线水平下和注射对照(非负载,n=5只小 鼠/组)或负载SF1‑AMPKα1‑DN...
(PGC-1α)和PPARa共同促进脂肪酸的氧化.我们采用Western Blot方法检测小鼠肝脏中p-AMPK和Lipinl的表达.结果显示RSV用药后p-AMPK表达明显增加,Lipin1表达明显降低,表明RSV对AFL小鼠的保护作用可能有AMPK和Lipinl的参与.3.RSV改善乙醇油酸诱导的HepG2细胞脂质积累采用87 mmol/L乙醇和100 pmol/L油酸联合诱导HepG2细胞...
Metabolic control of mitochondrial biogenesis through the PGC-1 family regulatory network The PGC-1 family of regulated coactivators, consisting of PGC-1伪, PGC-1尾 and PRC, plays a central role in a regulatory network governing the transcriptio... Richard,C.,Scarpulla - Biochimica et ...
Velloso. Cold-induced PGC-1α expression modulates muscle glucose uptake through an insulin receptor/Akt-independent, AMPK-dependent pathway. Am J Physiol Endocrinol Metab 287: E686-E695, 2004. First published May 27, 2004; 10.1152/ ajpendo.00103.2004.—Peroxisome proliferator-activated receptor-γ...
摘要: 目的:观察电针对2型糖尿病小鼠体重,血脂及PPAR-γ/UCP2-AMPK-ACC信号通路的影响.方法:将C57BL/6小鼠随机分4组:正常组,2型糖尿病组(DM组),假性电针组(DM+SEA组),电针组(DM+EA组).评估小鼠体重,24 h食物消耗量,附睾脂肪重量及甘油三酯,胆固醇,高密度脂蛋白,低密度脂蛋白水平;取肝脏进行Western blot...