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cDNA libraries were qualitatively checked on the Bioanalyzer 2100 and quantified by fluorimetry using the Quant-iTTM PicoGreen® dsDNA Assay Kit (Thermo Fisher Scientific) on NanoDrop™ 3300 Fluorospectrometer (Thermo Fisher Scientific). Sequencing was performed on NextSeq500 platform, generating for...
Ubiquitinated proteins were affinity purified from cell lysates using the Signal SeekerTM ubiquitination assay kit and affinity purified RelA/p65 and ubiquitinated proteins analysed by Western blotting (B). Data are representative of two independent experiments. 4. Discussion We present evidence that ...
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The enzyme-digested products were separated into four ssDNA fragments, as the Tm value of the ssDNA was estimated to be 6 ◦C–14 ◦C (Tm = (4 ◦C) (G/C pairs) + (2 ◦C) (A/T pairs)). In the presence of TdT and dCTP, the four ssDNA fragments were all tailed by a...
Contents : Kant Yearbookdoi:10.1515/9783110196672.tocKant Yearbook