Removal of domain 8 of the beta subunit resulted in drastic reduction of the dissociation constant of the FRS x tRNA(Phe) complex. Neither the removal of arginine 2 of the beta subunit, which makes the only major contact between domains beta1-5 and the tRNA, nor the replacement of the ...
Mutations compromising the editing domain of alanyl-tRNA synthetase (AlaRS) have been shown to increase misacylation by Gly and Ser, leading to host cell disease pathology (Lee et al., 2006). For biotechnology applications, naturally occurring low-fidelity phenylalanyl-tRNA synthetases (PheRS) and...
The C-Ala Domain Brings Together Editing and Aminoacylation Functions on One tRNA. Protein synthesis involves the accurate attachment of amino acids to their matching transfer RNA (tRNA) molecules. Mistranslating the amino acids serine or... G Min,Chong,E Yeeting,... - 《Science》 被引量: ...
One of them, named Tx2.1, recognizes the anticodon and D-loop of tRNA via interaction with its stem I domain, similarly to the parental T-box, and selectively charges-biotinyl--phenylalanine (Bio-Phe) onto the 3′ end of the cognate tRNA in. We also demonstrated the ribosomal synthesis ...
Transfer RNAs fold into a globular two-domain, L-shaped structure with the amino acid acceptor terminus and anticodon at opposite ends. This chapter reports nine examples of sequence-specific aminoacylation of RNA oligonucleotides based on tRNA acceptor stems with both class I and class II tRNA ...
If MetRS conformational change is dependent on the inter-domain communication, the double mutant should prevent structural rearrangement and therefore prevent domain-domain communication, limiting tRNA aminoacylation, whereas the single mutants should retain the structure as well as the function of the ...
Escherichia coli encodes YadB, a protein displaying 34% identity with the catalytic core of glutamyl-tRNA synthetase but lacking the anticodon-binding domain. We show that YadB is a tRNA modifying enzyme that evidently glutamylates the queuosine residue, a modified nucleoside at the wobble position...
The minimal core of the QR domain is a 29-nt helix-loop RNA, which is also able to glutaminylate ATRib in trans. Its amino acid binding site is embedded in an 11-nt cluster that is adjacent to the loop that interacts with the ATRib domain. Our study shows that a minihelix-loop ...
Here, we report the production of the BlmIV and BlmIII NRPSs as an excised domain(s), module, or intact subunit form and biochemical characterizations of the resultant enzymes in vitro for their roles in BLM bithiazole biosynthesis. Our results (a) confirm that BlmIII-A(0) is a ...
Structural analyses of Methanococcus maripaludis enzyme [163] and Archaeoglobus fulgidus SepRS•tRNACys complex revealed extensive contacts with the tRNACys anticodon, but also reported successful engineering of the anticodon-binding domain to allow recognition of stop codon suppressor variants [164]. Int...