Several tRNAs can exist for each amino acid, but a given tRNA does not bind to more than one amino acid. The aminoacyl-tRNA synthetaseenzymerequires Mg2+and is highly specific both for the amino acid and for the
Aminoacyl tRNA synthetase An aminoacyl tRNA synthetase (aaRS) is an enzyme that catalyzes the esterification of a specific amino acid or its precursor
M. Structure of tyrosyl-tRNA synthetase refined at 2.3 A resolution. Interaction of the enzyme with the tyrosyl adenylate intermediate. J. Mol. Biol. 208, 83–98 (1989). CAS PubMed Google Scholar Schmidt, E. & Schimmel, P. Residues in a class I tRNA synthetase which determine ...
Interestingly, the activity of many ARSs in higher eukaryotes appears to be regulated by their presence in the cytoplasmic multi-tRNA synthetase complex (MSC), which is assembled in most cases via the appended domains and consists of nine ARSs [EPRS (which consists of ERS and PRS), DRS, IR...
In higher eukaryotic systems, eight ARSs and three ARS-interacting multi-functional proteins (AIMPs) form a multi-tRNA synthetase complex (MSC), which seems to contribute to cellular homeostasis. Of these, AIMPs are generally considered as non-enzyme factors, playing a scaffolding role during ...
In zebrafish, mutations in the SARS gene encoding seryl-tRNA synthetase are associated with altered vascular development17,18. An angiogenic role has recently been identified for the class II threonyl-tRNA synthetase (TARS for eukaryotes; ThrRS for prokaryotic orthologs) that is ...
(Erianietal.1990).TypeIenzymesusetheRossmanfoldwithintheiractivesites,andtype184M.YarusandM.IllangasekareIIenzymesuseanantiparallelsheet(Cusacketal.1990)knownelsewhereonlyinbiotinsynthetase(Artymiuketal.1994).Asoneconsequentialeffectofthesestructuraldifferences,typeIaaRSfirsttransferaminoacidtothe2 hydroxylofthe...
This invention relates to an isolated nucleic acid fragment encoding an aminoacyl-tRNA synthetase. The invention also relates to the construction of a chimeric gene encoding all or
tRNA- RNA molecules present in the cell (in at least 20 varieties, each variety capable of combining with a specific amino acid) that attach the correct amino acid to the protein chain that is being synthesized at the ribosome of the cell (according to directions coded in the mRNA) ...
This chapter focuses on aminoacyl-tRNA synthetase autoantibodies. The first non-Jo-1 antisynthetases (aS) was anti-PL-7 that was found to react with threonyl-tRNA synthetase (ThrRS). A remarkable feature of the aS is that antibodies to different members of the same enzyme family in ...