The 9 kDa polypeptide is composed of 75 amino acids with a molecular weight of 8,437. This protein exhibits no obvious sequence similarity to other proteins. The iron-sulfur protein fraction (IP) of complex I was separated into eight polypeptides, 75, 49, 30, 20, 18, 15, 13 kDa-A, ...
Immunoblot analysis with an anti-MCT1 polyclonal antibody suggested the presence of a 44-kDa protein in human circulation and N-terminal amino acid sequencing exhibited a stretch of 14 amino acids which is completely identical to MCT1. The unbound fractions from the GST-MCT1 fusion protein-...
Amino acids sequences of the T3 (2.4 kDa), T7 (3 kDa) and LF15 (1.8 kDa) peptides in comparison to tumstatin (28 kDa).Karryn, T. GraftonLyn, M. MoirJudith, L. BlackNicole, G. HansbroPhilip, M. HansbroJanette, K. Burgess...
The protein consists of 134 amino acids beginning with serine and ending with glutamic acid, and its N-terminal was blocked with acetate. The agreement of the present result with that obtained from nucleotide sequence analysis (Y. Ohyama et al. (1986) Biochem. Biophys. Res. Commun. 134, 51...
The open reading frame of 737 amino acids with a predicted molecular mass 79973 Da was confirmed by amino acid sequencing of peptides. The 80-kDa translation product is processed to the N-terminal 32-kDa enzyme, part of which is then covalently linked to actin. The estradiol dehydrogenase/...
Urine specimens ultrafiltered through 3-kDa-cutoff membranes had substantial biuret reactivity, but absorbance maxima were consistent with cross-reactive amino acids rather than peptides.Many compounds, including amino acids, amino acid derivatives, and dipeptides, cross-react in biuret assays. Our ...
The cDNA hybridized to a 2.4-kilobase retinal mRNA. The amino acid sequence derived from the cDNA sequence contains a glutamic acid-rich domain in which 68 of 109 amino acids are glutamic acid. In addition, this domain contains four repeats of a peptide of 11 amino acids and two repeats ...
Polyglutamylated peptide analysis could be complemented with absolute quantification of derivatized amino acids, including glutamic acid and glutamate, using GC–MS (Bollenbach and Tsikas 2020) and GC–MS/MS (Deutsch et al. 1999; Xu and Chance 2007). Nonetheless, the ability to access unique ...
www.nature.com/scientificreports OPEN Cell-free synthesis of functional human epidermal growth factor receptor: Investigation of ligand- received: 16 March 2016 independent dimerization in Sf21accepted:07September2016 Published:27September2016 microsomal membranes using non- canonical amino acids Robert B...
The endosome-to-Golgi or endocytic retrograde trafficking pathway is an important post-Golgi recycling route. Here we show that amino acids (AAs) can stimulate the retrograde trafficking and regulate the cell surface localization of certain Golgi membran