Souba WW, Pacitti AJ (1992) How amino acids get into cells: mechanisms, models, menus, and mediators. JPEN J Parenter Enteral Nutr 16:569‐578Souba W W, Pacitti A J. How amino acids get into cells: mechanisms, models, ...
The isotope pattern of the lower mass in the isotope pair was then subtracted from the full isotope pattern to obtain the correct peak heights of the higher mass peptide. RESULTS AND DISCUSSION The SILAC Strategy— Mammalian cells cannot synthesize a number of amino acids, therefore these “...
we applied stable isotope labelling with amino acids in cell culture (SILAC)-based quantitative proteomic approach to analyze differences in protein expression levels between parental HuH-7 and sorafenib-acquired resistance HuH-7 (HuH-7R) cellsin vitro, combined with an isobaric tags for relative and...
Phosphoproteome Analysis of HeLa Cells Using Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC) DE, IwahoriA, Zhong J, PandeyA(2005) Phosphoproteome analysis of HeLa cells using stable isotope labeling with amino acids in cell culture (SILAC). J... Ramars,Amanchy,Dario,... - ...
Little is known about the role of amino acids in cellular signaling pathways, especially as it pertains to pathways that regulate the rate of aging. However, it has been shown that methionine or tryptophan restriction extends lifespan in higher eukaryotes and increased proline or tryptophan levels...
This growth inhibition is accompanied by a decrease in intracellular pH (pHi), as well as a decrease in glycolysis. Elevated concentrations (mM) of some amino acids have been shown by others to protect cells exposed to two very different environmental stresses: nutrient starvation and hyper...
Any of a large number of compounds that are found in living cells, contain carbon, oxygen, hydrogen, and nitrogen, and join together to form proteins. ♦ About 20 amino acids are needed by animal cells to produce proteins, but only about half, callednonessential amino acids, can be produ...
1b), and amino acids most abundant in human food are glutamine/glutamate (median = 0.16 g/g total amino acids), asparagine/aspartate (median = 0.095 g/g total amino acids), leucine (median = 0.082 g/g total amino acids), and lysine (median = 0.076 g/g total amino ...
The use of Genetic Code Expansion technology to enable the site-specific incorporation of noncanonical amino acids (ncAAs) into proteins in living cells has transformed our ability to study biological processes and provided the exciting potential to develop modern medicines1,2,3,4,5,6,7,8. The...
Amino acid, any of a group of organic molecules that consist of a basic amino group, an acidic carboxyl group, and a unique organic side chain. The term amino acid is short for α-amino [alpha-amino] carboxylic acid. Examples of amino acids include glyci