氨基酸摄取能力检测试剂盒——Amino Acid Uptake Assay Kit货号:UP04 氨基酸摄取能力检测试剂盒 Amino Acid Uptake Assay Kit 商品信息 储存条件:0-5度保存 运输条件:室温 特点: ● 使用荧光显微镜、荧光酶标仪或流式细胞仪即可快速检测 ● 简便操作即可检测氨基酸摄取能力 ※注意:您选择的孔板类型会对实验结果产生...
不仅如此,癌细胞由于其自身糖酵解途径的亢进,造成乙酰辅酶A(Acetoacetyl-CoA)供应的减少,更加剧了对TCA循环来源之一的氨基酸的需求。基于此方面的研究发现,癌细胞中氨基酸转运体LAT1(L-type amino acid transporter 1)的表达明显增高,说明氨基酸的大量摄取是癌细胞的普遍特征之一。这一发现也有望成为癌症药物研发的新...
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Kidney slices of 7–17-week-old human fetuses were incubated for 90 min or less in Krebs-Ringer solution containing aminoisobutyric acid (AIB). Cell to medium ratios of AIB decrease with increasing fetal age. Uptake rate is slower than for mature human tissue. Both water content and ...
摘要: 1. Uptake of amino acids into the intracellular acid-soluble pool in Tetrahymena pyriformisfollows similar kinetics to those seen in mammalian cells. However, after an initial faster uptake phase,...DOI: 10.1007/BF00690413 被引量: 16 ...
Selenium metabolism in hepatocytes incubated with selenite, selenate, selenomethionine, Se-methylselenocysteine and methylseleninc acid and analysed by LC-... The aim of the present work was to estimate the amounts of selenium metabolites produced in a liver cell model. Five different selenium compou...
III ( 1996 ) Tundra plant uptake of amino acid and NH 4 + nitrogen in situ : plants compete well for amino acid N . Ecology , 77 , 2142 – ... JPSS Chapin,III - 《Ecology》 被引量: 796发表: 1996年 Tundra plant uptake of amino acid and NH4+ nitrogen in situ: Plants compete we...
To determine if the enzymes were affected by cisplatin treatment and/or amino acid deprivation, we measured enzyme activity in cell extracts, and found no change in activity in extracts derived from cisplatin-treated cells in the presence of 40 mM phosphate in the assay buffer, a phosphate ...
Radiolabelled amino-acid uptake assay AaSlif-expressing and control oocytes were conditioned in AA-free ND98 for 3–4 h and placed in 600 μl of 98 Na+ media containing 2 mM of final concentration of L-Arg and L-Lys with added 10 μl of 0.1 μCi μl−1 of L-[...
Compared with nontransformed human pancreatic ductal (HPDE) cells, PDAC cells exhibited significantly elevated BCAA uptake through solute carrier transporters, which were highly upregulated in pancreatic tumor tissues compared with normal tissues. Branched-chain amino-acid transaminase 2 (BCAT2) knockdown ...