N-terminal amino acidsequencingpyroglutamic acidSDS-PAGEelectroblottingObjective To develop a method for sequencing of amino acids at N-terminus of recombinant antibody with pyrog- lutamate blockage. Methods The pyroglutamate at N-terminus of ...
Racemization is generally faster for amino acids in terminally bond positions (especially the N-terminus), and slower for those that are internally bound; amino acids that have been completely released from their peptide chain to form free molecules racemize slowest. The progression of amino acids ...
amino acid drink amino acid group amino acid oxidase amino acid oxidases amino acid pool amino acid reagent Amino Acid Screen, Blood Amino Acid Screen, Urine amino acid sequence amino acid therapy Amino acids amino sugar amino sugars Amino terminus ...
It was shown that the structure of the N-terminal amino acid is determinative for the overall pyrazine production. Especially, the production of 2,5(6)-dimethylpyrazine and trimethylpyrazine was low in the case of proline, valine, or leucine at the N-terminus, whereas it was very high for...
2). The majority of the mutant proteins that carry single amino acid substitutions map near the N terminus of the dnaC coding region. These substitutions reside in predicted α-helical structures in a region distant from the Walker A and B boxes (30, 31). Some of the substituted amino ...
Alanine (A), proline (P), cysteine (C) or tyrosine (Y) was added to the N terminus of l-Cys, which modified the morphology of the resulting nanoparticle substantially. Extended Data Fig. 6 Characterization of surfaces inside the gaps of 432 helicoid III. a, Large-area SEM image of ...
aネオジム 钕 [translate] aa 156-amino-acid tandem repeat region in the N-terminus and a possible transmembrane region and tyrosine phosphorylation site in the C-terminus. 156氨基酸纵排重覆区域在N终点和一个可能的横跨膜区域和酚基乙氨酸磷酸化站点在C终点。 [translate] ...
Fig. 2: The N-terminus of CFL1 but not internal lysine is conjugated by SUMO1. a Diagram illustrating CFL1 SUMOylation identification using IP-MS. Endogenous CFL1 proteins were purified from Neuro-2a cells expressing His-SUMO1E93R through De-IP with anti-CFL1 antibody. IP products underwe...
The primary structure of eel calcitonin, containing 32 amino acid resudues, was elucidated using the reduced and aminoethylated derivative. Manual Edman degradation was employed from the N-terminus to the 31st residue in an average yield of about 90% and the C-terminal prolineamide was character...
In this report, we have discovered that p53-NTD spanning amino acid residues 1–125 (NTD125) interacted with WT p53 and stabilized its wild type conformation under physiological and elevated temperatures, bothin vitroand in cellular systems. NTD125 prevented irreversible thermal aggregation of heat ...