EMA requires a UV exposure to covalently bond to DNA; while this dye is effective and is not affected by intracellular treatments it is weakly fluorescent. Here we report on the optimization of a new class of viability dyes, the amine reactive viability dyes (ViD) as a dead cell exclusion ...
Reactive Dye TFP, SDP, and succinimidyl esters are preferred for the conjugation to proteins because they form a very stable amide bond between the dye or hapten and the protein. Isothiocyanates are also commonly used, although the resulting thiourea product ha...
An azide-reactive, water-soluble Cy7 dye for chemoselective labeling and detection of azido-tagged molecules via the Cu-free click reaction. 名称:DBCO-PEG4-Phosphoramidite 英文简介: DBCO-PEG4-Phosphoramidite is a reagent for incorporation of DBCO moiety onto an oligonucleotide for subsequent copper-...
The kit includes two types of specially modified polystyrene microspheres that have been optimized to react with the amine-reactive dye in the LIVE/DEAD Fixable dead cell stain kits. The ArC reactive beads (Component A) bind any of the amine-reactive dyes and provide a positive signal; the ...
View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes. Spectra Viewer Technical Data for CoraFluor™ 1, amine reactive M. Wt 1485.25 Storage Store at -20°C The technical data provided above is for guidance only. For batch specific data ...
The average de- gree of substitution can be estimated by using the following formula: A × MW protein = moles dye ∈ mg protein mL moles protein where A is the absorbance value of the oxidized dye at the ab- sorption maximum wavelength and, is the molar extinction coef- ficient of the ...
Dye fixation was found to be much higher than by conventional dyeing without pretreatment, even in the presence of a large amount of salt. The influence of pretreatment conditions on dye fixation, such as pad鈥揵ake variables and the concentration of poly(vinylamine chloride), has been studied...
According to the results obtained in this study, C392STP: i) can be synthesized through a low cost and simple pathway; ii) is an efficient building block for mono-labeling of oligomers with an efficiency as high as that of an amine-reactive Alexa Fluor 488 dye supplied in a commercial ...
Amine reactive dyeCell membrane integrityFishSpermFlow cytometryFluorescent dyes that binds irreversibly to cellular amines, come in several available emission spectra, and do not poses health concerns were used to evaluate membrane integrity in fish sperm cells. The objectives of the present study were...
A method for preparing phycobiliprotein/amine-reactive dye conjugates is disclosed in which the conjugates so prepared overcome the energy transfer/fluorescent quenching dilemma encountered in the use of prior art conjugates. A phycobiliprotein, for example, phycoerythrin or allophycocyanin, is conjugated...