Specifically, we show that the amine headgroups in ionizable lipids drive LNP immunogenicity by binding to Toll-like receptor 4 and CD1d and by promoting lipid-raft formation. Immunogenic LNPs favour a type-1 T-helper-cell-biased immune response marked by increases in the immunoglobulins IgG2c ...
core (different phospholipid acyl chain length); (iii) the nature of the ionizable group, Δp K bilayer p K water) of carboxylic acid or primary amine groups being opposite respectively ( Δp K a = + 2.5 for stearic acid and Δp K b = 4.9 for cysteamine, in DPPC in fluid phase)...