A method of using the reagent includes adding the reagent to a native protein mixture such that magnetic nanoparticles may conjugate to the protein with exposed primary amine groups in the native protein mixture. The method also includes separating the proteins with exposed primary amine group from ...
Trace-amine-associated receptors (TAARs), a group of biogenic amine receptors, have essential roles in neurological and metabolic homeostasis1. They recognize diverse endogenous trace amines and subsequently activate a range of G-protein-subtype signalling pathways2,3. Notably, TAAR1 has emerged as ...
Tuna, salmon and salami HI, TY, PEA, CAD, PUT, SPM, SPD 5% trichloroacetic acid + ELL in butanol/CHC13 (1:1) 40°C 60 min Gradient elution water/MeCN 37 Protein foods HI, CAD, PUT MeOH + cation-exchange with Amberlite IRC-50 60°C 60 min Gradient elution phosphate/MeOH/MeCN 38...
In both of these examples, the amines of one protein can be activated in isolation using the NHS-ester reactive group; when the second protein (bearing sulfhydryl groups) is added, it conjugates to each molecule of the first pro...
We present an approach for quantitative analysis of changes in the composition and phosphorylation of protein complexes by MS. It is based on a new class of stable isotope-labeling reagent, the amine-reactive isotope tag (N-isotag), for specific and quantitative labeling of peptides following ...
In contrast, the α-amino group at a protein's N-terminus usually has a pKaof ~7, so it can sometimes be selectively modified by reaction at near neutral pH. Furthermore, although amine acylation should usually be carried out above pH 8.5, the ...
Amine deprotonation triggers the oxidation reaction, with cleavage of the substrate NH and CH bonds occurring in an asynchronous fashion, as suggested by the multiple deuterium KIE on the rate constant for flavin reduction (kred). A pKa of 9.6 signifies the ionization of a group that facilitates...
Both drugs reduced meal protein intake signifIcantly more than PL (p < . 05); however, none of the subjects in any group decreased protein intake below the recom mended amount. Weight loss signifIcantly correlated with decreased snack CHO intake among the DF group (r = 0.485, P = .03...
- 《Journal of Protein Chemistry》 被引量: 27发表: 2000年 Oxidation of kynuramine by lentil seedling copper amine oxidase: demonstration of a single turnover mechanism in the apoenzyme Copper amine oxidase was shown to be able to oxidize kynuramine to the corresponding aldehyde, which ...
In both of these examples, the amines of one protein can be activated in isolation using the NHS-ester reactive group; when the second protein (bearing sulfhydryl groups) is added, it conjugates to each molecule of the first pro...