The catalytic promiscuity of LysEDH—which naturally catalyzes the oxidative deamination of l﹍ysine at the ゛mino group—was harnessed to create variants that exhibited alcohol dehydrogenase (ADH) and amine dehydrogenase (AmDH) activities. These ADH and AmDH activities could be switched by changing...
Cover Feature: Generation of Oxidoreductases with Dual Alcohol Dehydrogenase and Amine Dehydrogenase Activity (Chem. Eur. J. 10/2021)alcohol aminationalcohol dehydrogenasesamine dehydrogenasesbiocatalysisenzyme promiscuityAlcohol amination, alcohol dehydrogenases, amine dehydrogenases, biocatalysis, enzyme promiscuity...
, and these could also be re-used, with up to 81% residual activity after a third application [19]. Mutti and co-workers replacedGDHwithformate dehydrogenase(FDH), and performed the amination of 50mMpara-methoxy phenylacetone to give an 82% yield of the (R)-amine product with >99% e....
Coupling of immobilized AmDH and immobilized glucose dehydrogenase (GDH) for the asymmetric reductive amination of 1a gave (R)-2a in 99% ee and 74% yield, with a total turnover number (TTN) of 2940 for NADH recycling. Both immobilized enzymes showed good recyclability, retaining 81% ...
Catalytic enzyme histochemistry and biochemical analysis of dihydroorotate dehydrogenase/oxidase and succinate dehydrogenase in mammalian tissues, cells an... Dihydroorotate dehydrogenase (EC 1.3.3.1 or EC 1.3.99.11) catalyzes the fourth sequential step in the de novo synthesis of uridine monophosphate. In...
structures of three nat-AmDHs (AmDH4,MsmeAmDH andCfusAmDH) reveal the active site determinants of substrate and cofactor specificity and enable the rational engineering of AmDH4 for the generated activity towards pentan-2-one. Analysis of the three-dimensional catalytic site distribution among ...
histaminase (redirected fromAmine oxidase) Thesaurus Medical Encyclopedia Wikipedia Related to Amine oxidase:Lysyl oxidase his·tam·i·nase (hĭ-stăm′ə-nās′, -nāz′, hĭs′tə-mə-) n. An enzyme that catalyzes the inactivation of histamine and is found in the digestive system...
amine dehydrogenase wh84 exhibited better thermo-, pH and solvent stability with high activity recovery. (S)-2-aminobutan-1-ol was produced in up to 99% conversion and 99% ee in the continuous flow processes, and the space-time yields were up to 124.5 g L-1d-1. The continuous ...
The mechanism of amine oxidation catalyzed by d-arginine dehydrogenase (DADH) has been investigated using steady-state and rapid reaction kinetics, with pH, substrate and solvent deuterium kinetic isotope effects (KIE) as mechanistic probes, and computational studies. Previous results showed that 85–...
amine dehydrogenase activity, a robust amine dehydrogenase has been evolved with a single two-site library allowing for the direct production of (R)-1-(4-fluorophenyl)-propyl-2-amine from para-fluorophenylacetone with a kcat value of 6.85 s1 and a KM value of 7.75 mM for the ketone ...