It has been shown Microbial nitrogen-cycling in biochar-amended soil J Harter et al in several studies that biochar incorporation into soil can have diverse effects on soil quality, plant growth and greenhouse gas (GHG) emissions (Chan et al., 2008; Major et al., 2010; Singh et al., ...
qPCRs were setup in 96 well plates with a reaction volume of 10 μl. Reaction mixtures consisted of SsoAdvanced Universal SYBR Green Supermix or IQ SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA. USA), gene-specific primers and template DNA or cDNA. As standards, dilution ...