Vac is the inexplicable creative power of speech, whichgives form to the formless; gives birth to existence ; and, lends identity to objects by naming them. It is the facultywhich gives expression to ideas; calms the agitated minds; and, enables one to hear, see, grasp, and then describe...
Full size image Ammonia-oxidizing archaea From the library for archaeal amoA genes, 24 randomly drawn clones were sequenced. Phylogenetic analysis of the 18 unique archaeal amoA gene sequences (Fig. 3) showed that they clustered into seven OTUs, and that most of the sequences were homologous to...
in GenBank, and Phylogenetic trees of translated AioA amino acid sequences were generated using the neighbour-joining algorithms following the p-distance model in (Tamura et al.2007). The level of support for the phylogenies, derived from neighbour-joining analysis, was determined from 1000 boot...
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Full size table Discussion Anaerobic fungi reside in the rumen of herbivore and synergize with associated methanogenic archaea to decompose ingested plant biomass. Given the relative short retention time of lignin tissues in the rumen, researchers have long speculated that rumen fungus–methanogen consorti...
Full size image In order to initially regenerate DMPH4from 6,7-dimethyl-5,6,7,8-dihydropterine (DMPH2), which is an oxidized form of DMPH4, DPR fromE. coliK-12 (Vasudevan et al.1988) and PAH-L101Y-W180F were coexpressed in the sameE. colicells. DPR was selected for DMPH4regenera...
Full size image The highest production levels of 2,5-DKG reductase were obtained with the systemLb. plantarum/pSIP609, resulting in 104 U L-1without pH regulation and 262 U L-1with pH control at 6.5. Although formation of recombinant 2,5-DKG reductase byLb. plantarum(both pSIP603 and ...
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Full size table Result RNase A was expressed in BL21 (E. coli stain) and prepared in active form RNase A overexpression was detected using 15% SDS-PAGE in comparison with un-induced cultures as shown in Figure 1A. Additionally, the activity of RNase A in refolding solutionshowed proper total...
Full size table Material and methods To illustrate the protein structure of the StoTA wild type and its variants for the motif adaption, ChimeraX was employed (Goddard et al. 2018). The crystal structure of StoTA is available at the protein data bank (Berman et al. 2000), accession 6K8H...