Over 95% of mammalian multi-exon protein-coding genes have more than a single isoform30,31; therefore, many studies have sought to identify RNA molecules generated by AS, and deduce their influence on cell proteomes32,33,34. However, knowledge on the impact of different splicing variants on ...
The predominant type of AS found in moso bamboo was intron retention (38.70%), followed by alternative 5′ (31.86%) and 3′ (16.68%) splice sites and exon skipping (11.46%). The number of AS events in every gene was relevant to gene characteristics and gene expression. We also observed...
The SpE-protein gene spans 22 exons and the intron-exon structure (including exon phase) is highly conserved with that of the vertebrate loci (Fig. 1A; S1). As in vertebrates, the first exon of SpE-Can is noncoding; the start of translation is located in the second exon. We also ...
When possible, primers crossing the exon/intron boundary were chosen. Primer quality was additionally validated by melting amplicons generated by RT-qPCR as well as by amplicon size and purity determination using electrophoresis in 1% agarose gel. The conditions for RT-qPCR were as follows: pre-...