Mclaughlin & Harvey Ltd Utilising ourCleanlinebenching system complete with glass partitioning, this project entailed the outfitting of new laboratories across the £32mInnovation Centre & Campus Hubat the Roslin Institute in conjuction with aregional fitting team. ...
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Novocastra Laboratories Ltd, Newcastle-upon- Tyne, UK), CD68 (clone [PGM1]; 1:50; Dako, Glostrup, Denmark) and Amyloid β (Aβ) (1:12,000; Chemicon, Temecula, CA, USA), or the rabbit polyclonal antibody to phosphorylated pTDP-43 (pS409/410-2; 1:1500); Cosmo Bio Ltd, Tokyo, ...
Two specialist calibration laboratories, including our UKAS 17025 certified electrical test facility. Products You can also add an enquiry about our equipment to your basket. You can either specify the precise equipment you want, or let us have some details and we can help you to make an inform...
5’ Seg. (LGC Biosearch Technologie) that detects both NEAT1_1 and NEAT1_2 at 37 °C for 4 h. Following incubation, the cells were washed with the wash buffer A at 37 °C for 30 min and were then mounted using Hard Set Mounting Medium with DAPI (Vector Laboratories). The...
The sections were then incubated with a biotinylated secondary anti- mouse antibody (Vector Laboratories, Burlingame, CA) diluted 1:500 in PBST with 3% normal goat serum followed by incubation with the ABC-horseradish per- oxidase staining kit (Vector Laboratories, Burlingame, CA). Sections were ...
(shanghai)ltd.)、小鼠抗map2(ap20)(1:100,santacruzbiotechnology)在4℃温育一晚或两晚,最后与二抗:alexafluor488标记抗小鼠igg(1:1000,invitrogen)、cy3标记抗兔igg(1:500,jacksonimmunoresearch)、生物素化抗兔igg(1:200,vectorlaboratories、ba-10009)、生物素化抗小鼠igg(1:200,vectorlaboratories、ba-2000...
(Jackson ImmunoResearch Inc., West Grove, PA, USA) or Alexa Fluor 568-labeled goat anti-rat IgG antibody (Thermo Fisher Scientific). Nuclei were stained with Hoechst 33258 (Sigma-Aldrich) or Hard Set Mounting Medium with DAPI (Vector Laboratories, Inc., Burlingame, CA, USA). The cells ...
Cell monolayers at 50% confluency were used for transfection with plasmids using PolyJet reagent according to the manufacturer’s protocol (SignaGen Laboratories, Gaithersburg, MD, USA). In all, 3 µl of PolyJet was incubated with 1 µg of different plasmids in 0.2 ml of serum-...