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used: anti-CTIP ab18465 abcam, anti-CHAT AF3447 R&D Systems, anti-C21ORF2 ab167351 abcam, anti-NEK1 A304-569A Bethyl Laboratories. 4,6-diamidino-2-phenylindole (DAPI) was used to stain nuclei. Slides with sections were washed 3 × 15 min in 0.5% Triton-X-100 in PBS (PBS-T)...
SOD1G93A mice (B6.Cg-Tg(SOD1*G93A)1Gur/J) were obtained from Jackson Laboratories (Strain #: 004435). The Fn14−/− mouse model [36] was provided by Biogen. Both strains were on a C57BL/6 genetic background. Experimental procedures with live animals were authorized...
gov/24178749/ Rabbit anti-SOX9, Millipore Sigma AB5535 RRID:AB_2239761 Mouse anti-SQSTM1/P62, Abcam ab5641 RRID:AB_2747598 Mouse anti-SYT1, Synaptic Systems 105 011 RRID:AB_2619761 Goat Anti-Rabbit IgG-HRP, ThermoFisher 31462 RRID:AB_228338 Goat Anti-Mouse IgG-HRP, Vector Laboratories ...
Intermediate-length repeat expansions in ATAXIN-2 (ATXN2) are the strongest genetic risk factor for amyotrophic lateral sclerosis (ALS). At the molecular level, ATXN2 intermediate expansions enhance TDP-43 toxicity and pathology. However, whether this tr
A-839977 was from Tocris Bioscience (Bristol, UK). Mice. Adult B6.Cg-Tg(SOD1-G93A)1Gur/J mice expressing high copy number of mutant human SOD1 with a Gly93Ala substitution (SOD1-G93A) were originally obtained from Jackson Laboratories (Bar Harbor, ME, USA) and bred in indoor animal...
After incuba- tion with primary antibodies, cells were incubated with secondary antibodies conjugated with Alexa 488 and DyLight 405 (Jackson Immuno Research Laboratories, West Grove, PA). Immuno-stained cells were rehydrated with rehydration buffer [2X saline sodium citrate (SSC) and 50% ...
The sections were then incubated with a biotinylated secondary anti- mouse antibody (Vector Laboratories, Burlingame, CA) diluted 1:500 in PBST with 3% normal goat serum followed by incubation with the ABC-horseradish per- oxidase staining kit (Vector Laboratories, Burlingame, CA). Sections were ...
Cell monolayers at 50% confluency were used for transfection with plasmids using PolyJet reagent according to the manufacturer’s protocol (SignaGen Laboratories, Gaithersburg, MD, USA). In all, 3 µl of PolyJet was incubated with 1 µg of different plasmids in 0.2 ml of serum-...