Immunology An alphavirus NSP1 determinant modulates type I interferon induction THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL Mark T. Heise CruzCatherine ChouAlphaviruses are mosquito-transmitted viruses that can cause severe disease in both animals and humans. The host type I Interferon (IFN) ...
Here we present the cryo-electron microscopy structure of non-structural protein 1 (nsP1) of the alphavirus chikungunya virus, which is responsible for RNA capping and membrane binding of the viral replication machinery. The structure shows the enzyme in its active form, assembled in a monotopic...
A large part of the genome of the alphaviruses (about two-thirds, beginning from one-third into the genome and extending to the 5′-end) encodes nonstructural proteins that form the viral replicative complex nsP1, nsP2, nsP3, and nsP4). Structural proteins (core, E3, E2, 6K, and E1...
Modulation of type I IFN induction by a virulence determinant within the alphavirus nsP1 proteinModulation of type I IFN induction by a virulence determinant within the alphavirus nsP1 proteiningentaconnectVIROLOGY -NEW YORK-CruzC.C.SutharM
(nsp1, nsp2, nsp3, and nsp4) are encoded at the 5′ end; the five structural proteins, which include the capsid and E1 and E2 glycoproteins, are encoded at the 3′ end and translated from a subgenomic RNA. As a positive-sense RNA virus, the alphaviral genome is infectious, meaning...
We have shown that nsP1, one of the tour virus-encoded replicase proteins, is pahnitoylated and associated with these membranes when expressed alone in mammalian cells. Thus, nsPl may serve to anchor the alphavirus replicase. The site of palmitoylation was mapped to cysteines, 118-420 in ...
The positive sense alphavirus genome is translated to produce four non-structural proteins (nsP1–nsP4) that comprise the viral replication complex. A subgenomic RNA is also produced which encodes the five structural proteins (C, E3, E2, 6K, and E1) functioning in the assembly of progeny ...
cDNA plasmid was constructed by deleting the replicon genes nsP1, nsP2, nsP3 and almost entire nsP4 from the pSAV3-FL, resulting in 100 nt at nsP4 3′- end and intact internal untranslated regions and genes encoding the structural proteins plus 6K, termed Helper-6K (Fig. 1). ...
1. A DNA alphavirus structural protein expression cassette, comprising an inducible promoter and an alphavirus structural protein gene, wherein the promoter directs the expression of the alphavirus structural protein gene upon induction of the promoter within a cell, and wherein prior to induction ...
(2003) Functional analysis of nsP3 phosphoprotein mutants of Sindbis virus. J Virol 77:13106-16 Fata, Cori L; Sawicki, Stanley G; Sawicki, Dorothea L (2002) Modification of Asn374 of nsP1 suppresses a Sindbis virus nsP4 minus-strand polymerase mutant. J Virol 76:8641-9 Fata, Cori L...