Structure and replication of the alphavirus genome, p. 35-90. In S. Schlesinger and M. J. Schlesinger (ed.), Replication of Togaviridae and Flaviviridae. Plenum Press, New York.Strauss, E. G., and J. H. Strauss. 1986. Structure and replication of the alphavirus genome, p. 35-90. ...
Alphaviruses are enveloped viruses of the Togaviridae family whose genome consists of a positive-sense RNA of ~9–11kb with a 5′ cap structure and 3′ polyadenosine tail. From: Current Opinion in Virology, 2013 About this pageAdd to MendeleySet alert ...
The genusAlphavirusincludes several potentially lethal human viruses. Additionally, species such as Sindbis virus and Semliki Forest virus are important vectors for gene therapy, vaccination and cancer research, and important models for virion assembly and structural analyses. The genome encodes nine known...
However, alphavirus genomes are loosely packed: the native T = 4 virion containing a genome of 11.7 kb nt would have a Matthews coefficient (Vm) greater than 5 Å3/Da, which is much higher than, for example, ssRNA picornaviruses and bromoviruses [23]. Therefore, it is plausible that ...
Nonetheless, most alphavirus 3′ UTRs share a common core structure, with short repeated sequence elements (RSEs), and a 19–24 nucleotide CSE at the 3′ end of the genome immediately adjacent to the poly(A) tail (Pfeffer et al., 1998). Notably, RSEs of similar sequence are observed ...
1. A DNA alphavirus structural protein expression cassette, comprising an inducible promoter and an alphavirus structural protein gene, wherein the promoter directs the expression of the alphavirus structural protein gene upon induction of the promoter within a cell, and wherein prior to induction ...
Structure and replication of the alphavirus genome. ;The Togaviridae and Flaviviridae. S. S. Schlesinger and M. J. Schlesinger, eds. Plenum Press, New York. pp. 35-90. Xiong et al., Science, vol. 243, pp. 1188-1191 (1989). Report and Recommendations of the Panel to Access the ...
Alphaviruses are enveloped RNA viruses that contain several human pathogens. Due to intrinsic heterogeneity of alphavirus particles, a high resolution structure of the virion is currently lacking. Here we provide a 3.5 Å cryo-EM structure of Sindbi
et al. Subdomain cryo-EM structure of nodaviral replication protein A crown complex provides mechanistic insights into RNA genome replication. Proc. Natl Acad. Sci. USA 117, 18680–18691 (2020). CAS PubMed PubMed Central Google Scholar Hellström, K. et al. Partially uncleaved alphavirus ...
The 3′ terminal region of the antigenome has likewise been shown to play an integral role in positive-strand synthesis (Frolov et al., 2001), perhaps through the presence of a stem-loop structure corresponding to the inverse of that present in the 5′ end of the genome. Specific sequence...