均相光激化学发光免疫分析技术(amplified luminescent proximity homogeneous assay linked immunosorbent assay,AlphaLISA) 是一种以表面包被有亲和涂层的受体微球(acceptor beads) 和供体微球(donor beads) 为载体的均相免疫学检测技术。该技术灵敏度高、特异性强、背景影响低、样品要求低、耗费少、分析操作简单以及检测范围...
Alpha技术, 即放大化学发光亲和均相检测(Amplified Luminescent Proximity Homogeneous Assay, Alpha), 是一种以微珠为基础的检测技术, 包括供体微珠(Donor Beads)和受体微珠(Acceptor Beads)。结合于供体微珠上的分子(分子A)与结合于受体微珠上的分子(分子B)发生相互作用, 当两个微珠达到足够接近的状态时, 能够检...
方法采用针对HBx蛋白不同表位的两种单克隆抗体X36C和3F6-G10,将其中一种抗体X36C与受体微珠(Acceptor Beads)连接,另一种抗体3F6-G10生物素化,再将HCC病人FFPE总蛋白提取液、供体微珠(Donor Beads)及上述两种处理后的抗体在23℃共同孵育90min后,用EnSpire2300多功能酶标仪检测信号值。结果 FFPE蛋白提取液检测...
供体微珠 Unconjugated Donor Beads(未标记的供体微珠 1 mg 5 mg 50 mg Streptavidin Donor beads(亲和素标记的供体微珠 1 mg 5 mg 50 mg Glutathione Donor beads(谷胱甘肽标记的供体微珠 1 mg 5 mg 25 mg Nickel Chelate Donor beads(镍螯合物标记的供体微珠 1 mg 5 mg 25 mg 6762013 6762011 ...
In the AlphaLISA® SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence...
Page Product Information (2)Quality Control ...2 Analyte of Interest .. (3)Description of the AlphaLISA Assay ...3 Precautions . (3)Kit content: Reagents and
For this assay we chose donor beads that would interact specifically with the TUBEs technology we were evaluating. Initially we explored the optimal relationship between the GST-tagged TUBEs 1 and the glutathione donor beads. We found that the optimal μg/ml relationship of glutathione donor beads...
先介紹AlphaScreen原理(如下圖 示) , AlphaScreen® 其實是一種Amplified Luminescent Proximity Homogeneous Assay, 主要含有兩種Beads, 一為Donor另一為Acceptor, 當Donor Bead所帶有的A分子與 Acceptor Bead所帶有的B分子距離非常接近時, 可以使用 680 nm激發光去引 發其表面所帶有的光敏感物質(Photosensitizer) ...
HomogeneousAssay,主要含有兩種Beads,一為Donor另一為Acceptor,當DonorBead所帶有的A分子與 AcceptorBead所帶有的B分子距離非常接近時,可以使用680nm激發光去引發其表面所帶有的光敏感物質( Photosensitizer),使其催化周遭的氧分子形成活化態,產生效率可達每秒60000個氧分子活化態產生,藉此將訊號放 大,此活化態氧分子再與...
donor beads and acceptor beads,and detected by AlphaLISA with matrix standard curve.As a result,there are good linear relationships in the concentration range of 0.1-500 ng/mL,with correlation coefficients larger than 0.98.The cross-reactivities for clenbuterol,brombuterol,terbutaline were 143.3%,...