Here we examine the kinetics of alpha methyl mannose induced dissociation of yeast () that have been previously allowed to bind to concanavalin A (Con A) derivatized agarose beads, a model for eluting glycans from Con A beads in purification protocols and for releasing pathogens from human cells...
In over 50 separate trials using bound sets of roof and archenterons attached together, either 0.2 M or 1.0 M alpha methyl mannose was added to the pieces. The pieces remained together. However in 100% of the cases, manually separating the pieces in alpha methyl mannose at either molarity ...
Pratap JV, Jeyaprakash AA, Rani PG, Sekar K, Surolia A & Vijayan M (2002) Crystal structures of artocarpin, a Moraceae lectin with mannose specificity, and its complex with methyl-alpha-d-mannose: implications to the generation of carbohydrate specificity. J Mol Biol 317, 237-247....