PURPOSE:A bacterium producing alpha-glycerophosphate oxidase in a specific genus is cultured in an enriched medium containing ascorbic acid or its salt to increase the productivity of alpha-glycerophosphate oxidase markedly. CONSTITUTION:There are bacteria capable of producing alpha-glycerophosphate oxidase ...
An FAD-containing L-alpha-glycerophosphate oxidase has been purified to homogeneity from Streptococcus faecium. The purified protein exists as a dimer (sub... A Claiborne - 《Journal of Biological Chemistry》 被引量: 60发表: 1986年 The production of L-alpha-glycerophosphate during anaerobic glycolys...
An increase in the concentration of the mitochondrial L-α-glycerophosphate oxidase when the yeast is grown on glycerol has also been observed. A model is presented for the substrate specificity pattern of glycerol kinase of C. mycode... C Gancedo,JM Gancedo,A Sols - 《Febs Journal》 被引量...
PURPOSE:A bacterium producing alpha-glycerophosphate oxidase in a specific genus is cultured in an enriched medium containing ascorbic acid or its salt to increase the productivity of alpha-glycerophosphate oxidase markedly. CONSTITUTION:There are bacteria capable of producing alpha-glycerophosphate oxidase ...
PROCESS FOR THE PRODUCTION OF .alpha.-GLYCEROPHOSPHATE OXIDASE ABSTRACT OF THE DISCLOSURE An improved process for producing .alpha.-glycerophosph- ate oxidase is provided by growing a member of the family Lactobacillaciae in a medium comprising pyruvate and an inducer for .alpha.-glycerophosphate ...
PROBLEM TO BE SOLVED: To obtain a new L-α-glycerophosphate oxidase excellent in reactivity, and useful for clinically determining L-α-glycerophosphate in a serum or the like and for others.TODA ATSUSHI戸田 篤志HATTORI SHIZUO服部 静夫KAWAMURA YOSHIHISA...
PROBLEM TO BE SOLVED: To obtain a new L-α-glycerophosphate oxidase excellent in reactivity, and useful for clinically determining L-α-glycerophosphate in a serum or the like and for others. ;SOLUTION: This L-α-glycerophosphate oxidase has the following physicochemical properties. Activity: to ...
PROBLEM TO BE SOLVED: To obtain the subject new enzyme, having a partial mutation in an amino acid sequence of a protein having wild type L-αglycerophosphate oxidase activities derived from a microorganism, improved in stability and useful for measurement, etc., of neutral fats in blood serum...
The ability for bacteria to grow on glycerol has been linked to the genes responsible for encoding the glycerol facilitator (GlpF), glycerol kinase (GlpK), and either alpha-glycerophosphate oxidase (GlpO) or dehydrogenase (GlpD). GlpF aids in bringing glycerol into the cell, while GlpK ...
An FAD-containing L-alpha-glycerophosphate oxidase has been purified to homogeneity from Streptococcus faecium. The purified protein exists as a dimer (subunit Mr = 65,000); each subunit contains 1 mol of FAD. The enzyme contains no iron, as determined by atomic absorption spectroscopy. The ...