±-Amylase-like proteins were synthesized in a heterologous cell-free protein synthesizing system prepared from Escherichia coli. The proteins were precipitable with anti-±-amylase serum and detected only when RNA extracted from ±-amylase producing Bacillus subtilis cells was used as messenger. The ...
Expression of Alpha Amylase and Osmotin-Like Protein under Drought Stress in Sugar BeetYoussef, A. B.Ismail, Roba M.EL-Assal, S. E.Abdallah, Naglaa A.Journal of Agricultural Chemistry & Biotechnology
acrylamide quenching of the intrinsic tryptophan residues in the protein molecules indicate that at pH 3.0,the protein is in a partially unfolded conformation with more tryptophan residues exposed to the solvent as compared to the native conformation in the neutral pH.Calcium ion is required for the...