Identification by Mass Spectrometry of a New α-Tubulin Isotype Expressed in Human Breast and Lung Carcinoma Cell Lines The extensive C-terminal molecular heterogeneity of alpha- and beta-tubulin is a consequence of multiple isotypes, the products of distinct genes, that und... S Rao,F Aberg,...
In order to determine the efficiency of DA-iPSn differentiation, immunofluorescence staining was performed for the neuronal marker beta-III tubulin (TUBB3), and for markers of DA neurons, FOXA2 and tyrosine hydroxylase (TH). Furthermore, the ratios of FOXA2-positive 3 × SNCAand isogenic ...
Braak’s hypothesis states that sporadic Parkinson’s disease (PD) follows a specific progression of pathology from the peripheral to the central nervous system, and this progression can be monitored by detecting the accumulation of alpha-Synuclein (α-Syn) protein. Consequently, there is growing in...
which has previously been shown to suppress coronavirus replication in HAECs21. At days 1, 2, and 3 post infection cells were fixed and stained with antibodies against SARS-CoV-2 spike22, and α-tubulin as marker for ciliated cells at the apical surface23(Fig.4b). In infected, PBS...
Labeled aSyn, βIII tubulin, and DAPI are present in red, white, and blue, respectively. (e) Representative WB images of exogenous aSyn in total cell homogenate (T), in the cytosol (Cyt), and in cellular membrane (CM) fraction of exposed H4 cells show the presence of exogenous aSyn in...
Secondary peroxidase conjugated anti-goat and anti-mouse IgG were used at 1:1000 and 1:2000 dilutions, respectively, to visualize ACTN4 and b-tubulin. The histogram shows the levels of ACTN4 expression in DOV13 cultured on 2DCI and 3DCI relative to the b-tubulin expression. The data ...
Expression characteristics of H4_GE cells – kinetics of protein amounts. H4 cell lines were incubated with 10 µM tebufenozide for 0 to 5 days. For all samples, expression of housekeeper β-tubulin was used as reference. (A) Control Western blots of H4 cells were treated like the West...
Five gene products are differently regulated by both statins at mRNA as well as protein levels: Calsyntenin-2 (CLSTN2), lanosterol 14-alpha demethylase (CYP51A1), squalene synthase (FDFT1), isopentenyl-diphosphate delta-isomerase 1 (IDI1), and tubulin beta-2B chain (TUBB2B) (Supplementary ...
Alpha-synucleinopathies (α-synucleinopathies) such as Parkinson’s disease (PD), Parkinson’s disease dementia (PDD), dementia with Lewy bodies (DLB), and multiple system atrophy (MSA) are all characterized by aggregates of alpha-synuclein (α-syn), but
While genotoxic chemotherapeutic agents are among the most effective tools to combat cancer, they are often associated with severe adverse effects caused by indiscriminate DNA damage in non-tumor tissue as well as increased risk of secondary carcinogenes