The α-Tubulin rabbit polyclonal antibody was purchased from Beyotime (AF5012, Shanghai, China). Rabbit monoclonal antibodies against NF-κB p65 (4764), phospho-NF-κB p65 (3033), IκBα (4812), phospho-IκBα (2859), and MyD88 (4283) were purchased from Cell Signaling Technology (Danver...
We could detect reproducibly, the presence of phosphorylation of human α-Syn at serine 129 position (human-specific α-Syn antibody for transgenic expression) which increased with ageing (Fig. 4b). Presence of synucleinopathy was investigated using trypsin/Proteinase K digestion to identify the ...
Antibodies against HNF-4α, PKM1, Cyclin A, Cyclin B1 and α/β-Tubulin were purchased from Cell Signaling Technology (Beverly, MA, USA) at 1:1000 dilution. Antibody against β-actin (1:2000 dilution) was the product of Santa Cruz Biotechnology, Dallas, TX, USA. AFP (1:1,000 ...
To control for unwanted sources of variation, the relative expression of target proteins in various groups were normalized to that evaluated for β-actin or β-tubulin and were expressed as the relative fold to those of the WT or control group. 2.12. Statistical Analysis The data and ...
MET and the TAM kinases (TYRO3, AXL and MER); and XB002, a next-generation tissue factor (TF)-targeting ADC, administered via intravenous infusion and composed of a human monoclonal antibody (mAb) against TF that is conjugated to an auristatin-based microtubulin inhibitor (MTI) payload. It...
Representative Western blots showing a1 Na/KATPase, surviving, and tubulin expression in control siRNA and targeted iRNA transfected cells are shown. (f) Digoxin induced upregulation of survivin expression in the Hep3B cell line. Note an abrogation of digoxin effect on cells exposed to pNaKtide ...