One day prior to transduction, 10,000 Caco2 cells were seeded in 100 µl respective medium in a 96-well plate. For the addition of α1AT prior to infection, medium was replaced by 80 µl of serum-free medium and cells were incubated with serial dilutions of Prolastin (α1AT)...
This suggests that using the cut-off of 122 nM might produce some false negative results; it is dangerous to set too much store by ROC curve analysis when there are few positive results, in this case only one. Indeed, the major limitation of this study is the fact that the cohort of ...