结果一 题目 DMEM培养基与alpha-MEM 培养基有什么不同,alpha-MEM 该如何配制? 答案 DMEM浓度高,适于快速增值的细胞.自己配很麻烦,我们都是买的粉末,自己溶解,过滤.相关推荐 1DMEM培养基与alpha-MEM 培养基有什么不同,alpha-MEM 该如何配制?反馈 收藏 ...
网友 1 最佳答案 回答者:网友 DMEM浓度高,适于快速增值的细胞. 自己配很麻烦,我们都是买的粉末,自己溶解,过滤.我来回答 提交回答 重置等你来答 聚氧乙来自烯聚氧丙烯醚是什么类型的表亲燃还晶操杆万船德厂必面活性剂 异构醇聚氧乙烯聚晶氧丙烯醚 香茅醛的物性数据 芸香糖基本信息0 二乙画紧写运久乡又鲁台...
DMEM浓度高,适于快速增值的细胞.自己配很麻烦,我们都是买的粉末,自己溶解,过滤.
The 2 mL of Trypsin was inactivated by adding 8 mL of DMEM or MEM/F12 and transferring the total of 10 mL from the flask to a 15 mL Falcon tube. After centrifuging the cell solution at 400xg for 5 min and discarding the supernatant, the pellet was resuspended in 10 mL of medium ...
, 1× MEM-NEAA (Thermo Fisher Scientific), 0.5× GlutaMAX (Thermo Fisher Scientific), and 0.1 mM b-mercaptoethanol (Sigma). After 24 h, the medium was replaced by DeSR2 medium that is composed by DeSR1 plus 1× B27 (Thermo Fisher Scientific); the medium was refreshed every day ...
cells were switched back to basal differentiation medium and maintained with medium changes every 48 h. To enrich for myocyte populations, cardiac differentiation was treated for 96 h with glucose-free DMEM containing 5 mM sodiuml-lactate, 1% GlutaMAX and 1% penicillin-streptomycin, with med...
VSV-G from Dr. Lung-Ji Chang. Amphoteric HIV pseudoviruses containing the firefly luciferase gene were produced by transfecting 293 T cells with pHEF-VSV-G (10 μg) and pNL4-3. Luc.R-E- (10 μg) in 90-mm culture dishes. Briefly, plasmid DNA was diluted in Opti-MEM serum free ...
In detail, 3 μl mimic was mixed with 150 μl serum-free OptiMEM and 9 μl RNAiMax reagent was diluted in another 150 μl OptiMEM. Both solutions were mixed, incubated for 5 min at room temperature and then 250 13 7 90 B. Bartelt‑Kirbach, N. Golenhofen μl mixture/well ...
For the transfection, we used FuGENE HD transfection reagent (E2311, Roche) at a 1:3 DNA to reagent ratio in Opti-MEM I reduced serum medium (31985062, Gibco). Expression constructs for siRNA targeting human STT3B (EHU051881) or MAGT1 (EHU110431) were purchased from Sigma-Aldrich. ...
OptiMEM at a 2:1 ratio (2 μg PEI per 1 μg DNA), incubated for 20 min at RT and added to cells dropwise. 24 h post transfection, medium was replaced and cells were transduced with VSV-G-protein pseudotyped VSV encoding luciferase and GFP reporter gene (kindly provided by...