M112 protein, like M15 protein, can be activated by anti-beta-galactosidase but to a much higher level.doi:10.1016/0165-022X(81)90075-0J K WelplyA V FowlerI ZabinJournal of Biological ChemistryWelply JK, Fowler AV, Zabin I. Beta-galactosidase alpha-complementation. Overlapping sequences. J...
Alpha 补充试剂盒 β-半乳糖苷酶,Alpha Complementation Kit Beta-galactosidase产品形式保存建议 溶液常温保存于2-8℃/-20℃;细胞产品冷冻保存于-80℃以下低温环境其他 Molecular Depo是一个一站式品牌,其中包括抗体、蛋白、试剂盒、酶、染料、脂质、分子试剂等多种产品类型。专注于开发省时、经济高效的研究工具,以及...
Studies on beta-galactosidase alpha-complementation are reviewed. The isolation and structure of two beta-galactosidase fragments that form an enzymically active complex are described. One of these is a cyanogen bromide peptide from whole beta-galactosidase; the other is a dimeric protein from a lacZ...
Intracistronic complementation of N-terminally truncated beta-galactosidase mutants such as M15 by coexpressed alpha-peptide was originally discovered in Escherichia coli and exploited for plasmid cloning as the well-known blue-white screening method. We show here that alpha-complementation also works in...
In previous studies, a cyanogen bromide peptide derived from amino-acid residues 3-92 of beta-galactosidase (EC 3.2.1.23; beta-D-galactoside galactohydrolase) was shown to have alpha-donor activity in intracistronic alpha-complementation. We have now isolated the defective beta-galactosidase alpha-...
Individual mutants (-donors) were analyzed for their ability to restore (by -complementation) -galactosidase activity of the M15 deletion mutant (-acceptor), and the data were correlated to the structure of the -galactosidase tetramer. Most of the insertions were well tolerated, including many of...
beta-galactosidase Enzyme (EC 3.2.1.23, 1024aa) that cleaves the terminal β-galactose from ganglioside substrates and other glycoconjugates; encoded by the LacZ gene in E. coli, and widely used as a reporter gene, because a variety of coloured or fluorescent compounds can be produce...
Examples of labels for polypeptides include, but are not limited to, the following: radioisotopes or radionuclides (such as 35S or 131I) fluorescent labels (such as fluorescein isothiocyanate (FITC), rhodamine, lanthanide phosphors), enzymatic labels (such as horseradish peroxidase, beta-galactosidase...
beta-Galactosidase alpha-complementation. A model of protein-protein interaction. Studies on beta-galactosidase alpha-complementation are reviewed. The isolation and structure of two beta-galactosidase fragments that form an enzymically ... I Zabin - 《Molecular & Cellular Biochemistry》 被引量: 122发...
Insertional restoration of beta-galactosidase alpha-complementation. Gene 70:161-170.Cronan, J.E. Jr., Narasimhan, M.L., and Rawlings, M. 1988. Insertional restoration of β- galactosidase α-complementation (white-to- blue colony screening) facilitates assembly of synthetic genes. Gene 15:161...