This chapter discusses protein–protein interaction in the α-complementation system of β -galactosidase. α -complementation system in β -galactosidase system is a system of protein–protein interaction where
Enzyme activity is restored to two defective beta-galactosidase molecules (M15 protein lacking amino acid residues 11-41 and M112 protein lacking residues 23-31) by incubation with CNBr2 (residues 3-92 of beta-galactosidase). M15 and M112 proteins (alpha-acceptors) are dimers. Complemented enzyme...
Intracistronic complementation of N-terminally truncated beta-galactosidase mutants such as M15 by coexpressed alpha-peptide was originally discovered in Escherichia coli and exploited for plasmid cloning as the well-known blue-white screening method. We show here that alpha-complementation also works in...
Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations Suitable for blue/white screening by α-complementation of the β-galactosidase gene(Φ80Δ lacZM15) Resistance to phage T1 (fhuA2) Reduced recombination of cloned DNA (recA1) ...
Beta Galactosidase:Beta galactosidase is an enzyme which is capable of breaking glycosidic bonds between galactose and another sugar residue. It is important for the digestion of lactose.Answer and Explanation: Alpha complementation is a method of screening bacteria transformed by a plasmid. In alpha ...
Therefore, we explored the use of the β-galactosidase α-complementation-based fusion assay. In this assay, the alpha subunit of β-galactosidase is expressed in one cell population, while the omega subunit is expressed in another. If fusion between the cell populations occurs after they are ...
beta-galactosidase Enzyme (EC 3.2.1.23, 1024aa) that cleaves the terminal β-galactose from ganglioside substrates and other glycoconjugates; encoded by the LacZ gene in E. coli, and widely used as a reporter gene, because a variety of coloured or fluorescent compounds can be produce...
The success of an ingestible form of beta-galactosidase for in vivo use was not entirely surprising, since the ingested enzyme structurally and functionally duplicates beta-galactosidase existing within the human digestive system. There was initial concern as to whether an ingested form of beta-galact...
Examples of labels for polypeptides include, but are not limited to, the following: radioisotopes or radionuclides (such as 35S or 131I) fluorescent labels (such as fluorescein isothiocyanate (FITC), rhodamine, lanthanide phosphors), enzymatic labels (such as horseradish peroxidase, beta-galactosidase...
acids contain complementary sequences, although depending on the stringency of the hybridization, mismatches between bases are possible. The appropriate stringency for hybridizing nucleic acids depends on the length of the nucleic acids and the degree of complementation, variables well known in the art....