采用改进SDS-碱裂解法裂解细胞,离心吸附柱内的硅基质膜在高盐、低pH值状态下选择性地结合溶液中的质粒DNA Main steps 1.取1.5ml过夜培养的菌液,12000 rpm,离心30s,弃上清,尽可能倒干,收集菌体沉淀。 2.用100μl 溶液I(50mmol/L Tris-HCl pH8.0, 10mmol/L EDTA)重悬菌体沉淀,Tip吹打至彻底悬浮。 3.加...
2. Resuspend beads in 80 µL 2x SDS-sample buffer. 3. Boil beads for 5 min at +95°C to dissociate immunocomplexes from beads. 4. Sediment the beads by centrifugation at 2,500x g for 2 min at +4°C. 5. Analyze the supernatant in SDS-PAGE. ...
DatasheetSDS Details and Advantages Applications:WB,ELISA Reactivity:Rabbit IgG(Fcγ Fragment specific) Conjugate:HRP Advantages: High lot-to-lot consistency Increased sensitivity and higher affinity Animal-free production Summary Description: Anti-Rabbit IgG (Fcγ Fragment specific), AlpSdAbs®...