Mechanism studies showed that Alox12 depletion activated AMP-activated protein kinase (AMPK), leading to the inhibition of acetyl-CoA carboxylase1 (ACC1) enzyme activity and lipid synthesis. The recuse of the effects of Alox12 depletion using Alox12 metabolites 12S-HETE further confirmed that AMPK...
pECMV-Alox12-m-FLAG到货后请根据标签上文字判断产品属性(质粒/甘油菌) 1、收到质粒干粉后请先5000rpm离心1min,再加入20μl无菌水溶解质粒,室温放置1min; 2、从-80℃冰箱中取出相应的感受态,置于冰盒上解冻,并做好标记; 3、取2μl质粒加至100μl感受态中,冰浴30min; 4、42℃热激90s,再冰浴2min; 5、...
将肝脏组织石蜡切片放入65℃烘箱(30分钟)→二甲苯中(5分钟×3次)→100%酒精(1分钟)→90%酒精(1分钟)→70%酒精(1分钟)→蒸馏水洗→苏木素(5分钟)→自来水洗去切片上的浮色→1%盐酸酒精(1至3秒)→自来水洗数下→scott促蓝液(碳酸氢钠0.35g,硫酸镁2g,蒸馏水100ml)(1分钟)→自来水洗数下→伊红(1分钟)...
Furthermore, H2S reversed RSL3-induced Drp1 expression and mitochondrial damage, which lead to abnormal lipid metabolism as evidenced by altered expressions of ACSL4, FAS, ACC and CPT1 as well as higher acetyl-CoA contents in both cytoplasm and mitochondria. RSL3 promoted the protein expression ...