A microassay for NAD + -dependent xanthine dehydrogenase, based on measuring the production of NADH fluorometrically under anaerobic conditions, was developed and used to examine the actions of allyl alcohol on this activity in periportal and pericentral regions of the liver lobule. The oxygen-...
There was a good correlation (r = 0.99, P less than .001) between liver ADH activity and allyl alcohol-induced hepatotoxicity, measured as the release of sorbitol dehydrogenase into the bloodstream. Cytosolic free NAD+/NADH ratios in male rats were not significantly different among the three ...
it can be assumed that the decrease in the toxic effect of allyl alcohol and acrolein in the presence of GSH is associated with the addition of the thiol group of GSH to acrolein. Apparently, an important role in minimizing toxic effects belongs to thealdehyde dehydrogenaseoxidation of acrolein...
1988: Allyl Alcohol Selection for Lower Alcohol Dehydrogenase Activity in Nicotiana plumbaginifolia Cultured Cells Plant Physiology 86(1): 266-269 Ogita, A.; Nagao, Y.; Fujita, K-Ichi.; Tanaka, T. 2007: Amplification of vacuole-targeting fungicidal activity of antibacterial antibiotic polymyxin ...
Histochemically, a low but evident alcohol dehydrogenase activity could focally be demonstrated in the pancreatic acinar cell cytoplasm of the experimental and control rats. By electron microscopy, cytoplasmic lipid droplets, mitochondrial degeneration and necrosis were found in the acinar cells. Focal ...
About 80% of the cytosolic alcohol dehydrogenase activity and about 50% of the microsomal P450-content were destroyed. In vivo-inhibition of alcohol dehydrogenase by pyrazole or induction of aldehyde dehydrogenase by phenobarbital abolished AA-induced liver damage as well as glutathione depletio...
The role of alcohol dehydrogenase in the hepatic necrosis due to allyl alcohol was studied in two strains of the deermouse, Peromyscus maniculatus. Mice of the alcohol dehydrogenase-negative (AdhN) strain which lack alcohol dehydrogenase activity were resistant to allyl alcohol toxicity. In contrast...
The kinetics of the oxidation of allyl alcohol by yeast alcohol dehydrogenase (YADH) was studied with stopped-flow technique. The experimental results showed that the specific activity of YADH remained constant within theYADH concentration range ofO-16.4mg/dm3 and YADH exhibited maximum activity at...
Acrolein is formed by alcohol dehydrogenase from the hepatotoxin allyl alcohol. It can be conjugated with GSH or it can be further oxidized to the nontoxic agent acrylic acid by aldehyde dehydrogenase. Recently it was demonstrated (Ohno et al. 1985) that acrolein is responsible for the toxic ...
Also it is known that allyl alcohol is metabolized by the cytosolic enzyme alcohol dehydrogenase to acrolein (Rees and Tarlow 1967; Serafini-Cessi 1972). The latter is considered as one of the most important toxic metabolites responsible for the damage induced by allyl alcohol in liver and ...